通过脉冲场凝胶电泳对空肠弯曲菌利奥4型散发病例菌株和感染暴发菌株进行鉴别。

Discrimination by means of pulsed-field gel electrophoresis between strains of Campylobacter jejuni Lior type 4 derived from sporadic cases and from outbreaks of infection.

作者信息

Suzuki Y, Ishihara M, Saito M, Ishikawa N, Yokochi T

机构信息

Laboratory of Bacteriology, Aichi Prefectural Institute of Public Health, Japan.

出版信息

J Infect. 1994 Sep;29(2):183-7. doi: 10.1016/s0163-4453(94)90722-6.

DOI:10.1016/s0163-4453(94)90722-6

PMID:7806881

Abstract

The cleavage patterns of the genomic DNA in 42 strains of Campylobacter jejuni Lior type 4, obtained from sporadic cases and from outbreaks of infection were analysed by means of pulsed-field gel electrophoresis (PFGE). The cleavage of DNA with SmaI and SalI restriction enzymes showed 16 distinct fragment patterns in 20 sporadic isolates, indicating that they were heterogeneous. On the other hand, the patterns of 22 isolates derived from two outbreaks showed the same unique restriction patterns, respectively. PFGE may therefore prove useful for subclassifying strains of C. jejuni Lior type 4 and for discriminating between strains derived from sporadic cases and those derived from outbreaks of infection.

摘要

采用脉冲场凝胶电泳（PFGE）分析了从散发病例和感染暴发中分离得到的42株空肠弯曲菌Lior 4型菌株的基因组DNA切割模式。用SmaI和SalI限制性内切酶切割DNA，结果显示20株散发病例分离株中有16种不同的片段模式，表明它们具有异质性。另一方面，来自两次暴发的22株分离株分别显示出相同的独特限制性模式。因此，PFGE可能有助于对空肠弯曲菌Lior 4型菌株进行亚分类，并区分散发病例来源的菌株和感染暴发来源的菌株。