Huang Y S, Wainwright P E, Mills D E, Lin X, Horrobin D F
Efamol Research Institute, Kentville, Nova Scotia, Canada.
Proc Soc Exp Biol Med. 1993 Oct;204(1):54-64. doi: 10.3181/00379727-204-43634.
The present study examined the effects of supplementation of either 18:3n-3 or a mixture of its post-delta 6-desaturation metabolites, 20:5n-3/22:6n-3, in combination with either 18:2n-6 or its immediate delta 6-desaturation product, 18:3n-6, in the maternal diet (n-3 to n-6 ratio at 0.25) on brain, liver, heart, and kidney glycerophospholipid fatty acid composition in dams (B6D2F1 mice) and their 12-day-old suckling pups. As expected, n-3 and n-6 fatty acids competed for incorporation into tissue glycerophospholipids in both dams and their suckling pups. Feeding a 20:5n-3/22:6n-3 as compared with an 18:3n-3 rich diet increased the tissue levels of 20:5n-3 and 22:5n-3, whereas it decreased those of 20:3n-6 and 20:4n-6. Replacing 18:2n-6 with 18:3n-6 in the maternal diet increased significantly the levels of 18:3n-6, 20:3n-6, and 20:4n-6, whereas it reduced those of 20:5n-3. However, the effects of maternal dietary fats on tissue fatty acid compositions in pups were qualitatively similar to but quantitatively smaller than those in dams. The discrepancy might be due to differences in the composition of fatty acids taken up and synthesized by the dams and that transferred to the pups.
本研究考察了在母鼠饮食中添加18:3n-3或其δ-6去饱和后代谢产物的混合物20:5n-3/22:6n-3，并结合添加18:2n-6或其直接的δ-6去饱和产物18:3n-6（n-3与n-6比例为0.25），对母鼠（B6D2F1小鼠）及其12日龄哺乳幼崽的脑、肝、心脏和肾脏甘油磷脂脂肪酸组成的影响。如预期的那样，n-3和n-6脂肪酸在母鼠及其哺乳幼崽体内竞争掺入组织甘油磷脂。与富含18:3n-3的饮食相比，喂食20:5n-3/22:6n-3可提高组织中20:5n-3和22:5n-3的水平，而降低20:3n-6和20:4n-6的水平。在母鼠饮食中用18:3n-6替代18:2n-6可显著提高18:3n-6、20:3n-6和20:4n-6的水平，而降低20:5n-3的水平。然而，母鼠饮食脂肪对幼崽组织脂肪酸组成的影响在性质上与母鼠相似，但在数量上较小。这种差异可能是由于母鼠摄取和合成的脂肪酸组成与转移到幼崽体内的脂肪酸组成不同所致。
