Bowen R A, Clandinin M T
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Lipids. 2000 Apr;35(4):389-94. doi: 10.1007/s11745-000-536-8.
The objective of this study was to test the hypothesis that increasing maternal dietary 18:3n-3 by decreasing the 18:2n-6/18:3n-3 ratio will increase the 18:3n-3 and 22:6n-3 content of the whole body, liver, skin (epidermis, dermis, and subcutaneous tissue), epididymal fat pads, and muscles (arms and legs) of 2-wk-old rat pups. Sprague-Dawley dams at parturition were fed semipurified diets containing either a low (18:2n-6 to 18:3n-3 ratio of 24.7:1) or a high (1 8:2n-6 to 18:3n-3 ratio of 1.0:1) 18:3n-3 fatty acid content. During the first 2 wk of life, rat pups received only their dams' milk. Fatty acid composition of the pups' stomach contents (dams' milk), whole body, brain, liver, skin, epididymal fat pads, and muscles was determined. The stomach fatty acid composition of 18:3n-3 reflected the dams' diet. The content of 18:3n-3 in whole body, brain, liver, skin, epididymal fat pads, and muscles was significantly (P< 0.05) greater in rat pups fed the high compared with the low 18:3n-3 fatty acid diet. The 22:6n-3 content of the whole body, brain, skin, epididymal fat pads, and muscles was not quantitatively different in rat pups fed either the low or high 18:3n-3 fatty acid diet. The 20:5n-3 and 22:5n-3 content of the whole body, skin, and epididymal fat pads was significantly increased in rat pups fed the high compared with the low 18:3n-3 fatty acid diet. High content of 18:3n-3 was found in the skin of rat pups fed either a low or high 18:3n-3 fatty acid diet. These findings demonstrate that high maternal dietary 18:3n-3 significantly increases the 18:3n-3 but not the 22:6n-3 content of the whole body, brain, skin, epididymal fat pads, and muscles with approximately 39 and 41% of the whole body 18:3n-3 content being deposited in the skin of suckling rat pups fed either the low or high 18:3n-3 diet, respectively.
通过降低18:2n-6/18:3n-3比例来增加母体饮食中的18:3n-3，会提高2周龄幼鼠全身、肝脏、皮肤（表皮、真皮和皮下组织）、附睾脂肪垫以及肌肉（手臂和腿部）中18:3n-3和22:6n-3的含量。分娩时的斯普拉格-道利母鼠被喂食半纯化日粮，其18:3n-3脂肪酸含量要么低（18:2n-6与18:3n-3的比例为24.7:1），要么高（18:2n-6与18:3n-3的比例为1.0:1）。在出生后的前2周，幼鼠仅食用母鼠的乳汁。测定了幼鼠胃内容物（母鼠乳汁）、全身、大脑、肝脏、皮肤、附睾脂肪垫和肌肉的脂肪酸组成。幼鼠胃中18:3n-3的脂肪酸组成反映了母鼠的饮食情况。与低18:3n-3脂肪酸饮食组相比，高18:3n-3脂肪酸饮食组幼鼠全身、大脑、肝脏、皮肤、附睾脂肪垫和肌肉中18:3n-3的含量显著更高（P<0.05）。低18:3n-3脂肪酸饮食组和高18:3n-3脂肪酸饮食组幼鼠全身、大脑、皮肤、附睾脂肪垫和肌肉中22:6n-3的含量在数量上没有差异。与低18:3n-3脂肪酸饮食组相比，高18:3n-3脂肪酸饮食组幼鼠全身、皮肤和附睾脂肪垫中20:5n-3和22:5n-3的含量显著增加。低18:3n-3脂肪酸饮食组和高18:3n-3脂肪酸饮食组幼鼠的皮肤中均发现18:3n-3含量较高。这些研究结果表明，母体饮食中高含量的18:3n-3可显著提高幼鼠全身、大脑、皮肤、附睾脂肪垫和肌肉中18:3n-3的含量，但不会提高22:6n-3的含量，在低18:3n-3饮食组和高18:3n-3饮食组的哺乳幼鼠皮肤中，全身18:3n-3含量分别约有39%和41%沉积其中。
