al-Othman A A, Rosenstein F, Lei K Y
Department of Nutrition and Food Science, University of Arizona, Tucson 85721.
Proc Soc Exp Biol Med. 1993 Oct;204(1):97-103. doi: 10.3181/00379727-204-43640.
This study examined the influence of dietary copper status on the in vivo hepatic fatty acid synthesis and the incorporation of nascent fatty acids into various hepatic lipid classes. Fifty weanling male Sprague-Dawley rats were assigned to two dietary treatments, copper deficient (5.4 nmol/g of diet) and copper adequate (102 nmol/g of diet). After 7 weeks of treatment, rats were injected with 0.111 MBq of [1-14C] acetate (1.85 GBq/mM)/100 g body wt through the femoral vein. Five rats from each treatment were sacrificed at 3, 6, 9, 12, and 24 min after injection. Radioactivities of nascent fatty acid samples were used to determine relative rates of fatty acid synthesis and their assembly into triacylglycerols and phospholipids. Linear increases were observed up to 12 min after injection for total hepatic fatty acid synthesis and their assembly into triacylglycerols and phospholipids for both treatments. In addition, 46% and 30% of total fatty acid synthesized were assembled into triacylglycerols and phospholipids, respectively, for both groups. Furthermore, hepatic fatty acid synthesis and assembly into triacylglycerols and phospholipids were enhanced more than 2-fold by copper deficiency when the data were expressed as per liver per 100 g body weight.
本研究考察了膳食铜状态对体内肝脏脂肪酸合成以及新生脂肪酸掺入各种肝脏脂质类别的影响。将50只断乳雄性Sprague-Dawley大鼠分为两种膳食处理组，即缺铜组（膳食含铜量为5.4 nmol/g）和铜充足组（膳食含铜量为102 nmol/g）。处理7周后，通过股静脉给大鼠注射0.111 MBq的[1-14C]乙酸盐（1.85 GBq/mmol）/100 g体重。在注射后3、6、9、12和24分钟，处死每种处理组中的5只大鼠。利用新生脂肪酸样品的放射性来测定脂肪酸合成及其组装成三酰甘油和磷脂的相对速率。两种处理组在注射后12分钟内，肝脏总脂肪酸合成及其组装成三酰甘油和磷脂的量均呈线性增加。此外，两组中合成的总脂肪酸分别有46%和30%组装成了三酰甘油和磷脂。此外，当数据以每100 g体重肝脏为单位表示时，缺铜使肝脏脂肪酸合成及其组装成三酰甘油和磷脂的能力增强了2倍多。
