McGuire W L, De La Garza M, Chamness G C
Cancer Res. 1977 Mar;37(3):637-9.
Standard dextran-coated charcoal (DCC) and sucrose gradient centrifugation assays for estrogen receptor were compared in a series of human breast cancer tissues. From a quantitative standpoint the results were remarkably similar. A simplified version of the DCC assay compared to the sucrose gradient assay yielded acceptable results. We conclude that, in spite of the lack of specificity controls inherent in the sophisticated standard assays, the simplified DCC assay might be useful if the biopsy specimen is too small to provide the number of aliquots for a standard DCC assay or sufficient protein for a sucrose gradient analysis. It also might be useful in research laboratories attempting to develop assays for multiple receptors or other constituents in a single tumor biopsy specimen.
在一系列人类乳腺癌组织中，对用于雌激素受体检测的标准葡聚糖包被活性炭（DCC）法和蔗糖密度梯度离心法进行了比较。从定量角度来看，结果非常相似。与蔗糖密度梯度法相比，DCC法的简化版本也能得出可接受的结果。我们得出结论，尽管复杂的标准检测方法本身缺乏特异性对照，但如果活检标本太小，无法提供用于标准DCC检测的等分试样数量，或者无法提供用于蔗糖密度梯度分析的足够蛋白质，那么简化的DCC检测方法可能会有用。它在试图针对单个肿瘤活检标本开发多种受体或其他成分检测方法的研究实验室中可能也很有用。
