Burfeind P, Belgardt B, Szpirer C, Hoyer-Fender S
Department of Human Genetics, University of Göttingen, Germany.
Eur J Biochem. 1993 Sep 1;216(2):497-505. doi: 10.1111/j.1432-1033.1993.tb18168.x.
Outer dense fibres are located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. Here we describe the isolation and genomic organization of a rat gene encoding a cysteine-proline-rich outer dense fibre protein. The cDNA sequence of rts 5/1 and its expression pattern have already been published [Burfeind, P. & Hoyer-Fender, S. (1991) Dev. Biol. 148, 195-204]. There exist two different genes in the rat genome, rts 5/1 major and rts 5/1 minor. Rts 5/1 major consists of two exons interrupted by one intron of about 3.8 kb. Exon 1 and rts 5/1 minor contains a deletion of 120 bp, without destroying the open reading frame, which is flanked by short direct repeats, 15 bp in length. The first two nucleotides of the intronic sequence were identified as GA and, therefore, do not agree with the donor consensus sequence. From the analysis of mouse x rat cell hybrids, the rts 5/1 major gene has been assigned to chromosome 7. By immunoblotting and immunocytochemistry, it was demonstrated that the isolated gene encodes a major protein of rat sperm outer dense fibres.
外周致密纤维位于哺乳动物精子尾部中段和主段的轴丝外侧，可能有助于维持精子尾部的被动弹性结构和弹性回缩。在此，我们描述了一个编码富含半胱氨酸-脯氨酸的外周致密纤维蛋白的大鼠基因的分离及其基因组组织。rts 5/1的cDNA序列及其表达模式已发表[Burfeind, P. & Hoyer-Fender, S. (1991) Dev. Biol. 148, 195 - 204]。大鼠基因组中存在两个不同的基因，即rts 5/1主要基因和rts 5/1次要基因。rts 5/1主要基因由两个外显子组成，中间被一个约3.8 kb的内含子隔开。外显子1和rts 5/1次要基因包含一个120 bp的缺失，未破坏开放阅读框，其两侧为长度为15 bp的短直接重复序列。内含子序列的前两个核苷酸被鉴定为GA，因此与供体共有序列不一致。通过对小鼠×大鼠细胞杂交体的分析，rts 5/1主要基因已被定位到7号染色体。通过免疫印迹和免疫细胞化学方法，证明分离出的基因编码大鼠精子外周致密纤维的一种主要蛋白质。
