使用离散动力学分析仪对淀粉酶活性进行自动比浊测定。

Automated turbidimetry of amylase activity by use of a discrete kinetic analyzer.

作者信息

Malkus H, Ibanez J, Castro A, DiCesare J L

出版信息

Clin Chem. 1977 Jan;23(1):122-4.

Abstract

We adopted an automated turbidimetric rate method for determining amylase activity to the KA-150 Kinetic Analyzer. In the method, an insoluble amylopectin substrate is used with activity determined by the rate of decrease in turbidity. Run-to-run CV for 59 samples with activities up to 400 units (arbitrary amylase units per 100 ml of sample) was 2.8%. A comparison with a similar method, performed by nephelometry, for 104 sera, showed a correlation coefficient (r) of 0.992, with a slope of 1.02. In an additional comparison with an amyloclastic method, for 52 sera r was 0.997, with a slope of 1.01. Day-to-day precision for control sera with activities near the upper limit of normal (279 and 216 units) averaged 2.5% during two months. Measured and calculated activity were linearly related to well above the upper limit of normal (normal range, 60-200 arbitrary units), showing a deviation from linearity of about 10% at 450 units. Commercial reagents available for the Perkin-Elmer Model 91 Amylase Lipase Analyzer can be used with the KA-150.

摘要

我们采用自动比浊速率法在KA - 150动力学分析仪上测定淀粉酶活性。在该方法中，使用不溶性支链淀粉底物，通过浊度降低速率来测定活性。对59份活性高达400单位（每100 ml样品中的任意淀粉酶单位）的样品进行批间变异系数（CV）为2.8%。对104份血清采用与类似的比浊法进行比较，相关系数（r）为0.992，斜率为1.02。在与淀粉分解法的另一次比较中，对52份血清r为0.997，斜率为1.01。在两个月期间，对活性接近正常上限（279和216单位）的对照血清进行的日间精密度平均为2.5%。测量活性和计算活性在远高于正常上限（正常范围为60 - 200任意单位）时呈线性相关，在450单位时显示出约10%的线性偏差。可用于珀金埃尔默91型淀粉酶脂肪酶分析仪的商用试剂可与KA - 150一起使用。