Alves A M, Tanuri A, de Almeida D F, von Krüger W M
Laboratório de Fisiologia Celular, Universidade Federal do Rio de Janeiro, Brazil.
Exp Parasitol. 1993 Sep;77(2):246-53. doi: 10.1006/expr.1993.1081.
The stability of zymodemes in clonal cultures of Trypanosoma cruzi derived from strain Y was followed. Reversible changes in the isoenzyme electrophoretic mobility pattern from type A to types B and C were observed after subculturing of cloned cultures in medium of different composition or after passage in newborn mice. Type A zymodeme was observed in clones grown in blood-containing media, while types B and C were found in clones and subclones grown in media progressively less rich in nutrients (10 and 5% fetal calf serum, respectively) and containing no blood. The change in zymodeme from type A to type B or C was associated with loss of infectivity, which could be recovered by passages in newborn mice. Parasites infective for mice always showed zymodeme A. Simultaneously with zymodeme change from type A to types B and C there is a decrease in the specific activity of G6PD and 6PGD.
对源自Y株的克氏锥虫克隆培养物中酶型的稳定性进行了跟踪研究。在将克隆培养物接种于不同成分的培养基中传代培养后，或在新生小鼠体内传代后，观察到同工酶电泳迁移率模式从A型可逆地转变为B型和C型。在含血培养基中生长的克隆中观察到A型酶型，而在营养成分逐渐减少（分别为10%和5%胎牛血清）且不含血的培养基中生长的克隆及亚克隆中发现了B型和C型酶型。酶型从A型转变为B型或C型与感染性丧失有关，通过在新生小鼠体内传代可恢复感染性。对小鼠具有感染性的寄生虫始终显示为A型酶型。在酶型从A型转变为B型和C型的同时，葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）的比活性降低。
