Winter C H, Hoving E B, Muskiet F A
Central Laboratory for Clinical Chemistry, University Hospital Groningen, Netherlands.
J Chromatogr. 1993 Jun 23;616(1):9-24.
Human milk triglycerides (TGs) were separated into 14 fractions by silver ion high-performance liquid chromatography (HPLC) with light-scattering detection (LSD). Subsequent fractionation by reversed-phase HPLC-LSD resulted in 75 subfractions. The major 48 were analysed by gas chromatography for their intact TG and fatty acid (FA) compositions. Using a constrained non-linear optimization computer program, the FA compositions and abundances of 170 different TG combinations were calculated. The major two, 16:0/18:1/18:1 (11.8 mol%) and 16:0/18:1/18:2 (10.0 mol%), were almost twice as abundant as expected from random FA distribution. The apolarities of 16:0 and 18:0 seem to be modulated by unsaturated FAs (18:1, 18:2) or medium-chain FAs (MCFAs; 8:0-12:0). MCFAs were present in 19 mol%. Of these, 7.2 mol% are likely to harbour stereospecific structures with an MCFA at sn-3, 16:0 or 14:0 at sn-2, and 18:1 or 18:2 at sn-1. These structures are excellent substrates for lingual and gastric lipases, producing products that assist in duodenal TG lipolysis.
人乳甘油三酯（TGs）通过带有光散射检测（LSD）的银离子高效液相色谱（HPLC）分离为14个组分。随后通过反相HPLC-LSD进行分级分离，得到75个子组分。对主要的48个子组分进行气相色谱分析，测定其完整的TG和脂肪酸（FA）组成。使用一个受限的非线性优化计算机程序，计算了170种不同TG组合的FA组成和丰度。主要的两种组合，16:0/18:1/18:1（11.8摩尔%）和16:0/18:1/18:2（10.0摩尔%），其丰度几乎是随机FA分布预期值的两倍。16:0和18:0的非极性似乎受到不饱和脂肪酸（18:1、18:2）或中链脂肪酸（MCFAs；8:0 - 12:0）的调节。MCFAs的含量为19摩尔%。其中，7.2摩尔%可能具有立体特异性结构，在sn-3位为MCFA，在sn-2位为16:0或14:0，在sn-1位为18:1或18:2。这些结构是舌脂肪酶和胃脂肪酶的优良底物，产生有助于十二指肠TG脂解的产物。
