Stoeckle M Y, Guan L, Riegler N, Weitzman I, Kreiswirth B, Kornblum J, Laraque F, Riley L W
Cornell University Medical College, New York, New York.
J Infect Dis. 1993 Oct;168(4):1063-5. doi: 10.1093/infdis/168.4.1063.
Isoniazid resistance in Mycobacterium tuberculosis is associated with lack of catalase-peroxidase activity. A recent study showed that some isoniazid-resistant M. tuberculosis strains have a complete deletion of the gene (katG) encoding this enzyme. To examine what proportion of clinical isolates of M. tuberculosis have katG deletion, katG sequences in 80 randomly selected isolates from New York City were analyzed. Polymerase chain reaction was used to amplify a 282-bp segment of M. tuberculosis katG and showed that 35 (90%) of 39 isoniazid-sensitive and 31 (76%) of 41 isoniazid-resistant strains contained katG sequences (P > .1). Ten multidrug and high-level isoniazid-resistant strains with identical restriction fragment length polymorphism patterns were also analyzed. All were found to have katG sequences. These findings suggest that mechanisms other than complete deletion of katG are involved in isoniazid resistance among most clinical isolates of M. tuberculosis from New York City.
结核分枝杆菌对异烟肼耐药与缺乏过氧化氢酶-过氧化物酶活性有关。最近一项研究表明，一些耐异烟肼的结核分枝杆菌菌株完全缺失了编码该酶的基因（katG）。为了检测结核分枝杆菌临床分离株中katG缺失的比例，对从纽约市随机选取的80株分离株的katG序列进行了分析。采用聚合酶链反应扩增结核分枝杆菌katG的一段282 bp片段，结果显示，39株异烟肼敏感菌株中有35株（90%）以及41株异烟肼耐药菌株中有31株（76%）含有katG序列（P>.1）。还分析了10株具有相同限制性片段长度多态性模式的耐多药和高水平耐异烟肼菌株。发现所有菌株均有katG序列。这些发现表明，在纽约市大多数结核分枝杆菌临床分离株中，除katG完全缺失外，其他机制也参与了异烟肼耐药。
