Jaber M, Rattan A, Kumar R
Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
J Clin Pathol. 1996 Nov;49(11):945-7. doi: 10.1136/jcp.49.11.945.
It has been reported recently that isoniazid resistant strains of Mycobacterium tuberculosis have lost the katG gene which encodes the catalase-peroxidase enzyme. A 35 mer oligonucleotide probe specific for the katG gene of M tuberculosis, 3' end-labelled with digoxigenin, was constructed and hybridised with DNA extracted from 26 clinical isolates of M tuberculosis under high stringency conditions. Twenty two of these isolates were resistant to 0.2 microgram/ml isoniazid and 20 to 1.0 microgram/ml isoniazid. Semiquantitative detection of catalase did not show any discrimination between isoniazid sensitive and resistant strains. The katG gene was present in all clinical strains of M tuberculosis. Therefore, complete deletion of the katG gene does not seem to be the mechanism of isoniazid resistance in M tuberculosis strains isolated from patients in India.
最近有报道称，结核分枝杆菌的异烟肼耐药菌株已失去编码过氧化氢酶-过氧化物酶的katG基因。构建了一种针对结核分枝杆菌katG基因的35聚体寡核苷酸探针，其3'端用地高辛标记，并在高严格条件下与从26株结核分枝杆菌临床分离株中提取的DNA杂交。这些分离株中有22株对0.2微克/毫升异烟肼耐药，20株对1.0微克/毫升异烟肼耐药。过氧化氢酶的半定量检测未显示异烟肼敏感和耐药菌株之间有任何区别。所有结核分枝杆菌临床菌株中均存在katG基因。因此，katG基因的完全缺失似乎不是从印度患者中分离出的结核分枝杆菌菌株异烟肼耐药的机制。
