Cenini P, Berhe N, Hailu A, McGinnes K, Frommel D
Armaure Hansen Research Institute, Addis Ababa, Ethiopia.
J Infect Dis. 1993 Oct;168(4):986-93. doi: 10.1093/infdis/168.4.986.
Serum cytokine levels and mononuclear cell subpopulations in the spleen and peripheral blood of patients with visceral leishmaniasis before and after antimony therapy were analyzed. The percentages of activated monocytes/macrophages, T cells, and possibly B cells; of gamma/delta T cell receptor (TCR)-bearing T cells; of CD4- CD8- alpha/beta TCR-bearing T cells; and serum levels of tumor necrosis factor-alpha (TNF alpha), interferon-gamma (IFN-gamma), and interleukin-6 (IL-6) were high in patients with active visceral leishmaniasis. The proportion of both helper and suppressor CD4+ cells and of cells with NK and cytotoxic T phenotypes were depressed. Successful chemotherapy normalized these parameters with the exception of activated monocytes. Thus, the impaired cell-mediated immunity in human Leishmania donovani infection is primarily due to a decrease in the proportion and possibly the activity of helper CD4+ cells, while suppressor cells do not seem to play a relevant role. TNF alpha, IL-6, and IFN-gamma may prove to be useful markers for monitoring response to therapy.
分析了内脏利什曼病患者在锑剂治疗前后血清细胞因子水平以及脾脏和外周血中的单核细胞亚群。活动性内脏利什曼病患者中，活化单核细胞/巨噬细胞、T细胞以及可能的B细胞的百分比；携带γ/δT细胞受体（TCR）的T细胞的百分比；携带CD4 - CD8 - α/βTCR的T细胞的百分比；以及肿瘤坏死因子-α（TNFα）、干扰素-γ（IFN-γ）和白细胞介素-6（IL-6）的血清水平均较高。辅助性和抑制性CD4⁺细胞以及具有NK和细胞毒性T表型的细胞比例均降低。成功的化疗使这些参数恢复正常，但活化单核细胞除外。因此，人类杜氏利什曼原虫感染中细胞介导的免疫受损主要是由于辅助性CD4⁺细胞比例降低以及可能其活性降低，而抑制性细胞似乎未发挥相关作用。TNFα、IL-6和IFN-γ可能被证明是监测治疗反应的有用标志物。
