在接合过程中，RP4的oriT处DNA切割存在两种不同机制的间接证据。
Indirect evidence for two distinct mechanisms of DNA cleavage at the oriT of RP4 during conjugation.
作者信息
Waterman S R, Hackett J, Manning P A
机构信息
Department of Microbiology and Immunology, University of Adelaide, Australia.
出版信息
Plasmid. 1993 Jul;30(1):79-81. doi: 10.1006/plas.1993.1035.
PMID:8378447
Abstract
Mobilization of a potential Campylobacter shuttle vector from Escherichia coli K-12 into C. hyointestinalis generated a small plasmid harboring a hybrid oriT nick region. This is presumed to have resulted from the cleavage and religation of the oriT of RP4 and the putative nick region of the shuttle vector. These data imply that two distinct DNA cleavage reactions occur at the oriT site of RP4 during conjugal transfer.
摘要
一种潜在的弯曲杆菌穿梭载体从大肠杆菌K-12转移至猪肠弯曲杆菌后，产生了一个携带杂交oriT切口区域的小质粒。据推测，这是由RP4的oriT和穿梭载体假定的切口区域的切割和重新连接导致的。这些数据表明，在接合转移过程中，RP4的oriT位点会发生两种不同的DNA切割反应。
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