Morisaki N, Xu Q P, Koshikawa T, Saito Y, Yoshida S, Ueda S
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Scand J Clin Lab Invest. 1993 Jul;53(4):347-52. doi: 10.3109/00365519309086626.
In culture, rabbit aortic smooth muscle cells (SMC) from an atheroma differed phenotypically from SMC from normal media (M-SMC) in their growth rate, secretion of SMC-derived growth factor (SDGF), and metabolism of acetylated low density lipoproteins (a-LDL). The factor responsible for this in vivo phenotypic change of SMC was investigated in vitro. After preincubation of M-SMC with 0.1-10 U ml-1 of tumour necrosis factor-alpha (TNF) for 1-3 days, the cells grew faster than control cells and secreted a substantial amount of SDGF. The population doubling time and secretion of SDGF were inversely correlated. Moreover, after preincubation with TNF, the SMC metabolized [125I]a-LDL, unlike control M-SMC. These findings show that TNF can modulate the phenotype of SMC and suggest that it is important in the pathogenesis of atherosclerosis.
在培养过程中，取自动脉粥样硬化斑块的兔主动脉平滑肌细胞（SMC）在生长速率、平滑肌细胞衍生生长因子（SDGF）分泌以及乙酰化低密度脂蛋白（a-LDL）代谢方面，其表型与取自正常中膜的SMC（M-SMC）不同。在体外研究了导致SMC这种体内表型变化的因素。用0.1 - 10 U/ml的肿瘤坏死因子-α（TNF）对M-SMC进行1 - 3天的预孵育后，细胞生长比对照细胞更快，并且分泌大量的SDGF。群体倍增时间与SDGF分泌呈负相关。此外，与对照M-SMC不同，经TNF预孵育后的SMC能够代谢[125I]a-LDL。这些发现表明TNF可调节SMC的表型，并提示其在动脉粥样硬化发病机制中具有重要作用。
