Pour P M, Permert J, Mogaki M, Fujii H, Kazakoff K
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
Am J Clin Pathol. 1993 Sep;100(3):223-30. doi: 10.1093/ajcp/100.3.223.
Nine human exocrine pancreatic adenocarcinomas were examined by serial sectioning and double- and triple-labeled immunohistochemical techniques with antibodies against chromogranin A, insulin, islet amyloid polypeptide, glucagon, somatostatin, pancreatic polypeptide, serotonin, pancreastatin, and neuron-specific enolase. The results were correlated with the stage of the disease, histologic characteristics of the tumors, and survival of the patients. Cells immunoreactive with most or all of the antibodies were found in all nine cases. Abnormal co-location of some hormones in the same cell and the lack of normal co-location of other hormones were found. Endocrine cells also were identified in the invasive regions of the cancer, including perineural spaces. Abnormality in the production and release of the peptide was indicated not only in the endocrine cells of exocrine cancer, but also in the islets near the cancer. Patients whose cancer contained many endocrine cells seemed to survive longer than those with tumors containing fewer endocrine cells. The overall data suggested that the observed abnormalities may contribute to the impaired glucose tolerance found in six of these patients.
采用连续切片以及针对嗜铬粒蛋白A、胰岛素、胰岛淀粉样多肽、胰高血糖素、生长抑素、胰多肽、5-羟色胺、胰抑制素和神经元特异性烯醇化酶的抗体进行双重和三重标记免疫组织化学技术，对9例人外分泌性胰腺腺癌进行了检查。将结果与疾病分期、肿瘤的组织学特征以及患者的生存率相关联。在所有9例病例中均发现了与大多数或所有抗体发生免疫反应的细胞。发现某些激素在同一细胞中异常共定位，而其他激素缺乏正常共定位。在癌症的浸润区域，包括神经周围间隙，也鉴定出了内分泌细胞。不仅外分泌癌的内分泌细胞，而且癌症附近胰岛中的肽产生和释放均存在异常。癌症中含有许多内分泌细胞的患者似乎比肿瘤中内分泌细胞较少的患者存活时间更长。总体数据表明，观察到的异常可能导致了其中6例患者出现糖耐量受损。
