从感染的伯基特淋巴瘤细胞系中对转化阴性的爱泼斯坦-巴尔病毒重组体进行标记拯救：一种用于分析转化必需基因的有用方法。
Marker rescue of a transformation-negative Epstein-Barr virus recombinant from an infected Burkitt lymphoma cell line: a method useful for analysis of genes essential for transformation.
作者信息
Marchini A, Kieff E, Longnecker R
机构信息
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
J Virol. 1993 Jan;67(1):606-9. doi: 10.1128/JVI.67.1.606-609.1993.
Abstract
A Burkitt lymphoma cell line infected in vitro with a transformation-defective mutant recombinant Epstein-Barr virus (EBV) was used to attempt marker rescue of transformation competence by transfection with cloned wild-type DNA. EBV replication was induced in the transfected cells, and wild-type EBV DNA recombined via flanking homologous sequences adjacent to the deletion, resulting in a virus which transformed primary B lymphocytes in vitro. This strategy should be useful for molecular genetic analysis of the role of part or all of any gene in cell growth transformation.
摘要
用一种体外感染了转化缺陷型突变重组爱泼斯坦-巴尔病毒（EBV）的伯基特淋巴瘤细胞系，通过用克隆的野生型DNA转染来尝试挽救转化能力标记。在转染细胞中诱导EBV复制，野生型EBV DNA通过缺失侧翼的同源序列进行重组，产生一种能在体外转化原代B淋巴细胞的病毒。该策略对于分析任何基因的部分或全部在细胞生长转化中的作用的分子遗传学研究应是有用的。
相似文献
1
Marker rescue of a transformation-negative Epstein-Barr virus recombinant from an infected Burkitt lymphoma cell line: a method useful for analysis of genes essential for transformation.
J Virol. 1993 Jan;67(1):606-9. doi: 10.1128/JVI.67.1.606-609.1993.
2
Epstein-Barr virus (EBV) recombinants: use of positive selection markers to rescue mutants in EBV-negative B-lymphoma cells.
J Virol. 1991 Apr;65(4):1701-9. doi: 10.1128/JVI.65.4.1701-1709.1991.
3
An Epstein-Barr virus with a 58-kilobase-pair deletion that includes BARF0 transforms B lymphocytes in vitro.
J Virol. 1994 Mar;68(3):1449-58. doi: 10.1128/JVI.68.3.1449-1458.1994.
4
Epstein-Barr virus recombinants from overlapping cosmid fragments.
J Virol. 1993 Dec;67(12):7298-306. doi: 10.1128/JVI.67.12.7298-7306.1993.
5
Epstein-Barr virus gene expression during primary B-lymphocyte infection, in transformed and Burkitt lymphoma-derived cell lines.
Curr Top Microbiol Immunol. 1986;132:251-6. doi: 10.1007/978-3-642-71562-4_38.
6
Second-site homologous recombination in Epstein-Barr virus: insertion of type 1 EBNA 3 genes in place of type 2 has no effect on in vitro infection.
J Virol. 1992 Feb;66(2):780-9. doi: 10.1128/JVI.66.2.780-789.1992.
7
Targeted gene disruption in Epstein-Barr virus.
Virology. 1992 Jul;189(1):253-65. doi: 10.1016/0042-6822(92)90701-p.
8
Epstein-Barr virus vectors for gene delivery to B lymphocytes.
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11334-40. doi: 10.1073/pnas.93.21.11334.
9
Influence of Burkitt's lymphoma and primary B cells on latent gene expression by the nonimmortalizing P3J-HR-1 strain of Epstein-Barr virus.
J Virol. 1989 Apr;63(4):1531-9. doi: 10.1128/JVI.63.4.1531-1539.1989.
10
Human immunodeficiency virus induction of malignant transformation in human B lymphocytes.
Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7635-9. doi: 10.1073/pnas.88.17.7635.
引用本文的文献
1
Epstein-Barr virus essential antigen EBNA3C attenuates H2AX expression.
J Virol. 2014 Apr;88(7):3776-88. doi: 10.1128/JVI.03568-13. Epub 2014 Jan 15.
2
Differential regulation of miR-21 and miR-146a by Epstein-Barr virus-encoded EBNA2.
Leukemia. 2012 Nov;26(11):2343-52. doi: 10.1038/leu.2012.108. Epub 2012 Apr 19.
3
Establishment of latent Epstein-Barr virus infection and stable episomal maintenance in murine B-cell lines.
J Virol. 2001 Mar;75(6):3016-20. doi: 10.1128/JVI.75.6.3016-3020.2001.
4
The genetic approach to the Epstein-Barr virus: from basic virology to gene therapy.
Mol Pathol. 2000 Oct;53(5):270-9. doi: 10.1136/mp.53.5.270.
5
Epstein-Barr virus vectors for gene delivery to B lymphocytes.
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11334-40. doi: 10.1073/pnas.93.21.11334.
6
Deletion of DNA encoding the first five transmembrane domains of Epstein-Barr virus latent membrane proteins 2A and 2B.
J Virol. 1993 Aug;67(8):5068-74. doi: 10.1128/JVI.67.8.5068-5074.1993.
7
Epstein-Barr virus recombinants from overlapping cosmid fragments.
J Virol. 1993 Dec;67(12):7298-306. doi: 10.1128/JVI.67.12.7298-7306.1993.
8
Epstein-Barr virus recombinant molecular genetic analysis of the LMP1 amino-terminal cytoplasmic domain reveals a probable structural role, with no component essential for primary B-lymphocyte growth transformation.
J Virol. 1994 Jul;68(7):4369-76. doi: 10.1128/JVI.68.7.4369-4376.1994.
9
An Epstein-Barr virus with a 58-kilobase-pair deletion that includes BARF0 transforms B lymphocytes in vitro.
J Virol. 1994 Mar;68(3):1449-58. doi: 10.1128/JVI.68.3.1449-1458.1994.
本文引用的文献
1
Identification of a rare Epstein-Barr virus variant that enhances early antigen expression in Raji cells.
Proc Natl Acad Sci U S A. 1983 May;80(9):2762-6. doi: 10.1073/pnas.80.9.2762.
2
Differences between laboratory strains of Epstein-Barr virus based on immortalization, abortive infection, and interference.
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4006-10. doi: 10.1073/pnas.71.10.4006.
3
Influence of the Epstein-Barr virus nuclear antigen EBNA 2 on the growth phenotype of virus-transformed B cells.
J Virol. 1987 May;61(5):1310-7. doi: 10.1128/JVI.61.5.1310-1317.1987.
4
Epstein-Barr virus-encoded protein found in plasma membranes of transformed cells.
J Virol. 1985 Sep;55(3):710-20. doi: 10.1128/JVI.55.3.710-720.1985.
5
Transformation by Epstein-Barr virus requires DNA sequences in the region of BamHI fragments Y and H.
J Virol. 1985 Aug;55(2):286-97. doi: 10.1128/JVI.55.2.286-297.1985.
6
Epstein-Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation.
Proc Natl Acad Sci U S A. 1989 Dec;86(23):9558-62. doi: 10.1073/pnas.86.23.9558.
7
Genetic analysis of immortalizing functions of Epstein-Barr virus in human B lymphocytes.
Nature. 1989 Aug 3;340(6232):393-7. doi: 10.1038/340393a0.
8
Epstein-Barr virus nuclear antigen 2 transactivates latent membrane protein LMP1.
J Virol. 1990 Jul;64(7):3407-16. doi: 10.1128/JVI.64.7.3407-3416.1990.
9
Epstein-Barr virus nuclear antigen 2 induces expression of the virus-encoded latent membrane protein.
J Virol. 1990 May;64(5):2126-34. doi: 10.1128/JVI.64.5.2126-2134.1990.
10
Epstein-Barr virus nuclear protein 2 mutations define essential domains for transformation and transactivation.
J Virol. 1991 May;65(5):2545-54. doi: 10.1128/JVI.65.5.2545-2554.1991.
Zotero 插件