Miller G, Robinson J, Heston L, Lipman M
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4006-10. doi: 10.1073/pnas.71.10.4006.
Biologic activities of extracellular Epstein-Barr virus (EB virus) from two laboratory strains, namely, P(3)J-HR-1 (P-H) from Burkitt lymphoma and B95-8 (B95) from infectious mononucleosis, were compared. Virus stocks from both sources contained approximately the same number of virions. Virus from the P-H line induced "early antigen" in six nonproducer EB virus genome carrier cell lines; virus from B95 did not induce "early antigen." Extracellular virus from B95 regularly caused lymphocytes from human umbilical cords to form continuous lines (immortalization); P-H virus did not cause primary cultures of human lymphocytes to grow continuously. B95 virus stimulated DNA synthesis as determined by rate of incorporation of [(3)H]thymidine into acid-insoluble material; P-H virus did not stimulate DNA synthesis. Pretreatment of lymphocytes with undiluted P-H virus inhibited immortalization and stimulation of DNA synthesis by B95 virus. The inhibitory properties of the P-H virus were sedimented at 100,000 x g and inactivated by heat and UV irradiation; interference by the P-H virus was neutralized by human serum with antibody to EB virus and not by antibody-negative human serum. The hypothesis most consistent with these results is that the P-H virus is defective in gene(s) needed for initiation of immortalization. We speculate that the absence of this gene allows early antigen to be expressed upon super-infection of nonproducer cell lines. The availability of two laboratory strains of EB virus which differ in biologic behavior provides starting material for analysis of the mechanism of lymphocyte immortalization by EB virus and of virus structural differences which affect immortalization.
比较了来自两种实验室毒株的细胞外爱泼斯坦-巴尔病毒（EB病毒）的生物学活性，这两种毒株分别是来自伯基特淋巴瘤的P(3)J-HR-1（P-H）和来自传染性单核细胞增多症的B95-8（B95）。来自这两种来源的病毒储备液中所含的病毒粒子数量大致相同。来自P-H株系的病毒可在六种非生产性EB病毒基因组载体细胞系中诱导“早期抗原”；来自B95的病毒则不能诱导“早期抗原”。来自B95的细胞外病毒能使来自人脐带的淋巴细胞形成连续细胞系（永生化）；P-H病毒不能使原代人淋巴细胞持续生长。通过[(3)H]胸苷掺入酸不溶性物质的速率测定，B95病毒可刺激DNA合成；P-H病毒则不能刺激DNA合成。用未稀释的P-H病毒预处理淋巴细胞可抑制B95病毒诱导的永生化和DNA合成刺激。P-H病毒的抑制特性在100,000×g离心时沉淀，可被加热和紫外线照射灭活；P-H病毒的干扰可被含有EB病毒抗体的人血清中和，而不能被抗体阴性的人血清中和。与这些结果最相符的假说是，P-H病毒在启动永生化所需的基因方面存在缺陷。我们推测，该基因的缺失使得在非生产性细胞系受到超感染时早期抗原得以表达。两种生物学行为不同的EB病毒实验室毒株的存在，为分析EB病毒使淋巴细胞永生化的机制以及影响永生化的病毒结构差异提供了起始材料。
