Burtonboy G, Lachapelle J M, Tennstedt D, Lamy M E
Ann Dermatol Venereol. 1978 Aug-Sep;105(8-9):707-12.
Viral particles can be visualized by electron microscopy using negative staining. Such an approach, widely used in research, provides a method for detecting virions which are present in clinical specimens. It is considered to be the method of choice in cases with suspicion of smallpox. Direct visualization has been systematically applied for the diagnosis of viral diseases in dermatology. Negative staining by pseudoreplication appears to be simple, rapid and efficient. It was thus possible to detect viruses in vesicle fluids, in scrapings and in crusts. During the course of this study 66 cases have been analyzed and viruses have been demonstrated in 49. If a skin lesion seems to be of viral origin, direct examination may confirm the clinical impression.
病毒颗粒可以通过负染色电子显微镜观察到。这种方法在研究中广泛使用，为检测临床标本中存在的病毒粒子提供了一种手段。在疑似天花的病例中，它被认为是首选方法。直接观察已被系统地应用于皮肤病学中病毒疾病的诊断。通过假复制进行负染色似乎简单、快速且有效。因此，可以在水疱液、刮屑和痂皮中检测到病毒。在本研究过程中，分析了66例病例，其中49例检测到了病毒。如果皮肤病变似乎是病毒源性的，直接检查可能会证实临床印象。
