用于动物病毒诊断的病毒分离、免疫荧光和电子显微镜检查的比较。
Comparison of virus isolation, immunofluorescence and electron microscopy for the diagnosis of animal viruses.
作者信息
Naeem K, Goyal S M
机构信息
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
出版信息
Microbiologica. 1988 Oct;11(4):355-62.
Results of 539 virus-positive samples from animals during the past three years were analyzed to determine the sensitivity of three different techniques i.e. virus isolation (VI), direct immunofluorescence (DFA), and electron microscopy (EM). The results showed that most of the positive cases were diagnosed with the help of all three techniques, either alone or in different combinations. While VI and DFA were more effective for the detection of bovine viral diarrhea, pseudorabies and infectious bovine rhinotracheitis viruses, EM was more sensitive for the detection of rota- and coronaviruses. The need for selective use of these tests for rapid diagnosis and the role of different factors affecting the sensitivity of these tests are discussed.
对过去三年中539份动物病毒阳性样本的结果进行了分析，以确定三种不同技术的敏感性，即病毒分离（VI）、直接免疫荧光（DFA）和电子显微镜（EM）。结果表明，大多数阳性病例是借助这三种技术中的一种或不同组合进行诊断的。虽然VI和DFA对检测牛病毒性腹泻、伪狂犬病和传染性牛鼻气管炎病毒更有效，但EM对检测轮状病毒和冠状病毒更敏感。本文讨论了为快速诊断而选择性使用这些检测方法的必要性以及影响这些检测方法敏感性的不同因素的作用。
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