在无内源性鼠白血病病毒的情况下大规模生产小鼠乳腺肿瘤病毒。

Large-scale production of mouse mammary tumor virus in the absence of endogenous murine leukemia virus.

作者信息

Benton C V, Harshman J S, Robinson O R, Shibley G P

出版信息

Appl Environ Microbiol. 1979 Jan;37(1):148-58. doi: 10.1128/aem.37.1.148-158.1979.

DOI:10.1128/aem.37.1.148-158.1979

PMID:83813

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243415/

Abstract

A system for the large-scale production and purification of mouse mammary tumor virus in the absence of detectable endogenous murine leukemia virus is described. By utilizing the Mm5mt/c1 cell line established from an adenocarcinoma of a C3H mouse, the continuous production of over 25,000 liters of mouse mammary tumor virus-containing tissue culture fluids has been achieved. By the strict adherence to well-defined tissue culture conditions, mammary tumor virus production was accomplished without the expression of murine leukemia virus. Various biochemical and immunological systems were established for the rapid and precise detection of the endogenous leukemia virus, the expression of which could be enhanced under conditions of culture stress.

摘要

描述了一种在不存在可检测到的内源性鼠白血病病毒的情况下大规模生产和纯化小鼠乳腺肿瘤病毒的系统。通过利用从C3H小鼠的腺癌建立的Mm5mt/c1细胞系，已实现连续生产超过25000升含小鼠乳腺肿瘤病毒的组织培养液。通过严格遵守明确的组织培养条件，在不表达鼠白血病病毒的情况下完成了乳腺肿瘤病毒的生产。建立了各种生化和免疫检测系统，用于快速、精确地检测内源性白血病病毒，在培养应激条件下其表达可能会增强。