小鼠乳腺肿瘤腹水细胞系中小鼠乳腺肿瘤病毒的体外产生

In vitro production of mouse mammary tumor virus in a mouse mammary tumor ascites line.

作者信息

Keydar J, Gilead Z, Hartman J R, Ben-Shaul Y

出版信息

Proc Natl Acad Sci U S A. 1973 Oct;70(10):2983-7. doi: 10.1073/pnas.70.10.2983.

DOI:10.1073/pnas.70.10.2983

PMID:4126729

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427152/

Abstract

An ascites line derived from a spontaneous mouse mammary carcinoma produces, on explantation and cultivation in vitro, large amounts of oncornavirus particles. The biochemical, biophysical, and electron microscopic characteristics of the virions are described. Molecular hybridization and immunological methods identify these virions as mouse mammary tumor virus.

摘要

从自发性小鼠乳腺癌中获得的腹水系，在体外接种培养时会产生大量肿瘤病毒颗粒。本文描述了这些病毒粒子的生化、生物物理及电子显微镜特征。分子杂交和免疫学方法鉴定这些病毒粒子为小鼠乳腺肿瘤病毒。