Yu M W, Chen C J
Comprehensive Cancer Center, School of Public Health, Columbia University, New York, New York 10032.
Cancer Res. 1993 Feb 15;53(4):790-4.
Serum samples of 9691 male adults had been collected and frozen for a prospective study of hepatocellular carcinoma in Taiwan. After an average follow-up period of 4.6 years, testosterone levels in the stored serum were measured by radioimmunoassay using commercial kits for 35 cases of newly developed hepatocellular carcinoma, 63 hepatitis B surface antigen-negative and 77 hepatitis B surface antigen-positive matched controls. Elevated testosterone levels were found to be associated with an increased risk of hepatocellular carcinoma. The association remained significant after the adjustment for effects of other hepatocellular carcinoma risk factors, including hepatitis B surface antigen carrier status, positivity of serum antibody to hepatitis C virus, cigarette smoking, alcohol drinking, past liver disease history, and dietary habits. The multivariate-adjusted relative risk of hepatocellular carcinoma for men with testosterone levels in the upper tertile was 4.1 (95% confidence interval = 1.3-13.2) compared with those having levels in the middle or lower tertiles (P = 0.016). The results consistent with those observed in animal experiments support the hypothesis that testosterone plays a role in the etiology of human hepatocellular carcinoma.
收集了9691名成年男性的血清样本并冷冻保存，用于台湾地区肝细胞癌的前瞻性研究。在平均随访4.6年后，使用商用试剂盒通过放射免疫分析法测定了35例新发生肝细胞癌患者、63例乙肝表面抗原阴性和77例乙肝表面抗原阳性匹配对照者储存血清中的睾酮水平。发现睾酮水平升高与肝细胞癌风险增加有关。在对其他肝细胞癌风险因素的影响进行调整后，这种关联仍然显著，这些因素包括乙肝表面抗原携带状态、丙肝病毒血清抗体阳性、吸烟、饮酒、既往肝病病史和饮食习惯。与睾酮水平处于中三分位数或低三分位数的男性相比，睾酮水平处于高三分位数的男性肝细胞癌的多变量调整相对风险为4.1（95%置信区间 = 1.3 - 13.2）（P = 0.016）。与在动物实验中观察到的结果一致，这些结果支持了睾酮在人类肝细胞癌病因中起作用的假说。
