Fabia R, Ar'Rajab A, Johansson M L, Willén R, Andersson R, Molin G, Bengmark S
Dept. of Surgery, Lund University, Sweden.
Scand J Gastroenterol. 1993 Feb;28(2):155-62. doi: 10.3109/00365529309096063.
The potential beneficial effect of exogenous administration of Lactobacillus on acetic acid-induced colitis was evaluated in the rat. Colitis was induced by instillation of 4% acetic acid for 15 sec in an exteriorized colonic segment. This produced uniform colitis with a threefold increase in myeloperoxidase (MPO) activity of the colonic tissue (an index of neutrophil infiltration) and a sixfold increase in plasma exudation into the lumen of the colon (mucosal permeability) as evaluated 4 days after acetic acid administration. Intracolonic administration of L. reuteri R2LC immediately after acetic acid administration, at a dose of 5 ml of 7 x 10(7) colony-forming units (CFU)/ml in two forms: either as pure bacterial suspension or as fermented oatmeal soup, prevented the development of colitis. Thus, the morphologic score, MPO activity, and mucosal permeability were almost normalized by Lactobacillus treatment. Initiating the treatment 24 h after acetic acid administration or using lower doses of 1 ml for 3 consecutive days resulted in a smaller protective effect. We conclude that exogenous administration of L. reuteri R2LC prevents the development of acetic acid-induced colitis in the rat.
在大鼠中评估了外源性给予罗伊氏乳杆菌对乙酸诱导的结肠炎的潜在有益作用。通过在一段外置结肠中滴注4%乙酸15秒来诱导结肠炎。这会产生均匀的结肠炎，在给予乙酸4天后评估，结肠组织的髓过氧化物酶（MPO）活性（中性粒细胞浸润指标）增加了三倍，血浆渗入结肠腔的量（黏膜通透性）增加了六倍。在给予乙酸后立即通过结肠内给药罗伊氏乳杆菌R2LC，剂量为5毫升含7×10⁷菌落形成单位（CFU）/毫升的两种形式：纯细菌悬液或发酵燕麦汤，可预防结肠炎的发展。因此，通过乳杆菌治疗，形态学评分、MPO活性和黏膜通透性几乎恢复正常。在给予乙酸24小时后开始治疗或连续3天使用1毫升的较低剂量，保护作用较小。我们得出结论，外源性给予罗伊氏乳杆菌R2LC可预防大鼠乙酸诱导的结肠炎的发展。
