Fry E, Acharya R, Stuart D
Laboratory of Molecular Biophysics, Oxford, England.
Acta Crystallogr A. 1993 Jan 1;49 ( Pt 1):45-55. doi: 10.1107/s0108767392005737.
The structure of foot-and-mouth disease virus (FMDV) strain O1 BFS 1860 has been determined to 2.9 A resolution using the molecular-replacement method [Acharya, Fry, Stuart, Fox, Rowlands & Brown (1989). Nature (London), 337, 709-716]. Crystals of the virus with average dimensions 0.12 x 0.06 x 0.12 mm belong to space group I23, a = 345 A with 1/12 of the icosahedral particle per asymmetric unit giving fivefold noncrystallographic redundancy. Oscillation diffraction photographs were collected at the SERC Synchrotron Radiation Source at Daresbury in accordance with strict disease security regulations. The ambiguity in particle orientation was resolved using a self-rotation function and starting estimates of the phases to 8 A were derived from the known structures of two picornaviruses similarly oriented in the I23 unit cell. The phases were refined and extended using iterative averaging and solvent flattening with the implementation of a simple automatic envelope-determination procedure to increase the phasing power available.
口蹄疫病毒（FMDV）O1 BFS 1860株的结构已通过分子置换法确定，分辨率达到2.9埃[阿查里亚、弗莱、斯图尔特、福克斯、罗兰兹和布朗（1989年）。《自然》（伦敦），337，709 - 716]。平均尺寸为0.12×0.06×0.12毫米的病毒晶体属于空间群I23，a = 345埃，每个不对称单位中有十二分之一的二十面体粒子，具有五重非晶体学冗余。振荡衍射照片是在达累斯伯里的SERC同步辐射源按照严格的疾病安全规定收集的。利用自旋转函数解决了粒子取向的模糊性，并根据在I23晶胞中取向相似的两种小核糖核酸病毒的已知结构，得出了至8埃的初始相位估计值。通过迭代平均和溶剂扁平化对相位进行了精修和扩展，并实施了一个简单的自动包络确定程序以提高可用的相位确定能力。
