Aznar J, Ojeda A, Torres M J, Palomares J C, Rodriguez-Pichardo A
STD Clinic, School of Medicine, University of Seville, Spain.
Genitourin Med. 1993 Feb;69(1):60-2. doi: 10.1136/sti.69.1.60.
We have carried out a prospective study of dual genitotropic human papillomavirus (HPV) infections by means of two different DNA detection methods in biopsy specimens obtained from patients who were examined for genital warts at the STD clinic of the School of Medicine in Seville, between January 1990 and December 1991.
100 patients with a clinical diagnosis of condilomata acuminata were seen during the study period. DNA of the genitotropic HPV 6/11, 16/18 and 31/33/35 was detected by an in situ hybridisation method in 75 (77%) of the 98 evaluable samples; one of the genotypes tested in 59 (61%) samples, and two or more genotypes tested in the remaining 16 (15%) samples. In 21 (98%) of the 23 negative samples by in situ hybridisation, we were able to detect DNA of genital HPV using a polymerase chain reaction amplification method (PCR). Among the 34 samples where PCR was applied we confirmed the presence of two different HPV genotypes in eight samples.
The frequency of dual infections with human genitotropic papillomavirus in genital warts was 8%, although we believe that this rate should be higher as we have not used the PCR method in all of the samples.
我们采用两种不同的DNA检测方法，对1990年1月至1991年12月间在塞维利亚医学院性病门诊接受尖锐湿疣检查的患者活检标本中的双重嗜性人乳头瘤病毒（HPV）感染进行了前瞻性研究。
在研究期间共观察到100例临床诊断为尖锐湿疣的患者。采用原位杂交法在98份可评估样本中的75份（77%）中检测到嗜性HPV 6/11、16/18和31/33/35的DNA；59份（61%）样本检测到一种基因型，其余16份（15%）样本检测到两种或更多种基因型。在23份原位杂交阴性样本中的21份（98%）中，我们采用聚合酶链反应扩增法（PCR）检测到了生殖器HPV的DNA。在应用PCR的34份样本中，我们在8份样本中证实存在两种不同的HPV基因型。
尖锐湿疣患者中双重嗜性人乳头瘤病毒感染的频率为8%，不过我们认为该比例应更高，因为我们并非对所有样本都采用了PCR方法。
