Sondermeijer P J, Claessens J A, Jenniskens P E, Mockett A P, Thijssen R A, Willemse M J, Morgan R W
Virological Research Department, Intervet International, Boxmeer, The Netherlands.
Vaccine. 1993;11(3):349-58. doi: 10.1016/0264-410x(93)90198-7.
Control of Marek's disease in the poultry industry has been successfully achieved for several decades by large-scale vaccination of day-old chickens with live herpesvirus of turkeys (HVT) strains. Several features of this virus including lack of pathogenicity and long-term immune protection due to a persistent viraemic infection made us decide to use HVT as a live viral vector for the expression of foreign antigens. Potential sites for the integration of foreign DNA in the unique short region of the HVT genome were identified by the insertion of a beta-galactosidase expression cassette. Vaccination trials with recombinant virus strains indicated that the marker gene was expressed and stably maintained during animal passage. Based on an insertion site mapping in one of the open reading frames of the unique short region, a general recombination vector was designed for the integration of foreign genes into HVT. Recombinant virus-directed expression of individual antigens from Newcastle disease virus was driven by a strong promoter element derived from the lung terminal repeat sequence of Rous sarcoma virus.
几十年来，通过给一日龄雏鸡大规模接种火鸡疱疹病毒（HVT）活毒株，家禽业成功实现了对马立克氏病的控制。这种病毒的几个特性，包括缺乏致病性以及由于持续性病毒血症感染而产生的长期免疫保护，促使我们决定使用HVT作为表达外源抗原的活病毒载体。通过插入β-半乳糖苷酶表达盒，确定了HVT基因组独特短区域中外源DNA整合的潜在位点。重组病毒株的疫苗接种试验表明，标记基因在动物传代过程中表达并稳定维持。基于在独特短区域的一个开放阅读框中的插入位点定位，设计了一种通用重组载体，用于将外源基因整合到HVT中。来自新城疫病毒的单个抗原的重组病毒定向表达由源自劳斯肉瘤病毒肺末端重复序列的强启动子元件驱动。
