Wirth U V
Department of Biological Chemistry, Junior College KZO, Dübendorf, Switzerland.
Virus Res. 1993 Jan;27(1):1-12. doi: 10.1016/0168-1702(93)90108-y.
Northern (RNA) blot analysis was used to compare immediate-early (IE) transcripts among strains of bovine herpesvirus type 1 and type 5 differing in neurovirulent potential. Results obtained from the neurovirulent strain N569 were compared to those from two non-neurovirulent strains, Jura and K22. Strain N569 expressed three major IE transcripts with similar size and transcription pattern as previously reported for strain Jura and K22 (Wirth et al., 1989, 1991, 1992), except for some differences in the 5' terminal sequence common to the alternative spliced IE transcripts IER4.2 and IER2.9. More significant differences were discovered for some minor IE transcripts. Those transcribed over joined genome ends during replicative phase were found to be drastically overexpressed for strain N569 under IE condition. Among them two very long IE transcripts were detected clearly for strain N569, however hardly recognized for strain Jura and K22. These two transcripts were found to be encoded in addition to the joined terminal HindIII genome fragments by the second HindIII fragment J from the left genome end.
采用Northern（RNA）印迹分析法，比较了1型和5型牛疱疹病毒不同神经毒力株之间的立即早期（IE）转录本。将神经毒力株N569的结果与两种非神经毒力株Jura和K22的结果进行了比较。N569株表达了三种主要的IE转录本，其大小和转录模式与先前报道的Jura株和K22株相似（Wirth等人，1989年、1991年、1992年），只是可变剪接的IE转录本IER4.2和IER2.9共有的5'末端序列存在一些差异。在一些次要的IE转录本中发现了更显著的差异。发现在复制期转录于连接基因组末端的那些转录本，在IE条件下N569株中显著过表达。其中，N569株清晰地检测到两个非常长的IE转录本，而Jura株和K22株几乎未检测到。发现这两个转录本除了由来自左基因组末端的第二个HindIII片段J编码连接的末端HindIII基因组片段外，还由其编码。
