Ohtani M, Yamashita T, Amano H, Kubo N, Kumazawa T
Department of Otolaryngology, Kansai Medical University, Osaka, Japan.
Acta Otolaryngol Suppl. 1993;500:46-9. doi: 10.3109/00016489309126178.
Caloric responses to intracellular calcium homeostasis of vestibular hair cells isolated from the guinea pig were examined. An increase in intracellular free calcium ion concentration ([Ca2+]i) in the isolated cells was observed by direct heat stimulation. The cells, dissociated enzymatically or mechanically, were stimulated by perfusion of solution, in the temperature range of 2.2-5.2 degrees C. The [Ca2+]i was analysed using a digital imaging microscope and the Ca2+ sensitive dye, Fura-2. There was an increase in [Ca2+]i in both Types I and II vestibular hair cells with this heat stimulation. The direct thermal influence on vestibular sensory cells is discussed in relation to mechanisms involved in caloric nystagmus.
研究了从豚鼠分离的前庭毛细胞对细胞内钙稳态的热刺激反应。通过直接热刺激观察到分离细胞内游离钙离子浓度（[Ca2+]i）增加。通过酶解或机械解离的细胞在2.2 - 5.2摄氏度的温度范围内通过溶液灌注进行刺激。使用数字成像显微镜和Ca2+敏感染料Fura-2分析[Ca2+]i。在这种热刺激下，I型和II型前庭毛细胞的[Ca2+]i均增加。结合温热性眼震的相关机制讨论了热对前庭感觉细胞的直接影响。
