小儿生殖细胞肿瘤中LINE-1逆转录转座子的表达
LINE-1 retrotransposon expression in pediatric germ cell tumors.
作者信息
Bratthauer G L, Fanning T G
机构信息
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000.
出版信息
Cancer. 1993 Apr 1;71(7):2383-6. doi: 10.1002/1097-0142(19930401)71:7<2383::aid-cncr2820710733>3.0.co;2-p.
BACKGROUND
Human LINE-1 (L1Hs) is a retrotransposon that is known to cause insertion mutations. Previous work demonstrated that at least 10% of adult testicular germ cell cancers expressed the L1Hs element.
METHODS
Pediatric germ cell tumors were assayed for L1Hs expression by in situ immunohistochemical methods using an antibody directed against one of the L1Hs-encoded proteins.
RESULTS
Approximately 10% of pediatric germ cell tumors express abundant amounts of the L1Hs protein. The element was expressed in 1 of 19 ovarian tumors, 1 of 20 testicular tumors, and 4 of 19 extragonadal tumors. The reactive cells in all cases appeared to be embryonal carcinoma or yolk sac tumor cells. None of 32 ovarian immature teratomas gave positive results, suggesting that more differentiated tissues do not express L1Hs abundantly.
CONCLUSIONS
It appears that neither the age nor sex of the patient, nor the location of the tumor, has a significant influence on the degree of L1Hs expression.
背景
人类长散在核元件1（L1Hs）是一种逆转座子，已知可导致插入突变。先前的研究表明，至少10%的成人睾丸生殖细胞癌表达L1Hs元件。
方法
采用针对L1Hs编码蛋白之一的抗体，通过原位免疫组化方法检测儿童生殖细胞肿瘤的L1Hs表达。
结果
约10%的儿童生殖细胞肿瘤表达大量L1Hs蛋白。该元件在19例卵巢肿瘤中的1例、20例睾丸肿瘤中的1例以及19例性腺外肿瘤中的4例中表达。所有病例中的反应性细胞似乎都是胚胎癌或卵黄囊瘤细胞。32例卵巢未成熟畸胎瘤均未得出阳性结果，提示分化程度更高的组织不会大量表达L1Hs。
结论
患者的年龄、性别以及肿瘤位置似乎均对L1Hs表达程度无显著影响。
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