Raible D W, McMorris F A
Wistar Institute, Philadelphia, Pennsylvania 19104.
J Neurosci Res. 1993 Feb 15;34(3):287-94. doi: 10.1002/jnr.490340305.
Oligodendrocytes, the glial cells specialized to synthesize myelin in the central nervous system, differentiate in primary rat brain cell cultures on a schedule similar to that observed in vivo. The schedule of oligodendrocyte differentiation and the rate of oligodendroglial progenitor cell proliferation in vitro are both modulated by 3',5'-cyclic AMP (cAMP). A 24-hour exposure to 1 mM N6,2'O-dibutyryladenosine 3',5'-cyclic monophosphate (dbcAMP) induced a wave of oligodendrocyte differentiation but inhibited proliferation of oligodendroglial progenitors, and reduced by 30-fold the proliferation of progenitors in response to platelet-derived growth factor (PDGF). When cells were grown in the presence of maximally stimulating concentrations of PDGF, the inhibitory effect of cAMP on progenitor cell proliferation was abolished while the stimulatory effect of cAMP on oligodendrocyte differentiation remained, demonstrating that these two cAMP-regulated events are independent.
少突胶质细胞是中枢神经系统中专门负责合成髓磷脂的神经胶质细胞，在原代大鼠脑细胞培养物中，其分化过程的时间安排与在体内观察到的情况相似。少突胶质细胞的分化进程以及少突胶质前体细胞在体外的增殖速率均受3',5'-环磷酸腺苷（cAMP）调节。暴露于1 mM N6,2'-二丁酰腺苷3',5'-环磷酸单酯（dbcAMP）24小时会引发一波少突胶质细胞分化，但会抑制少突胶质前体细胞的增殖，并且使前体细胞对血小板衍生生长因子（PDGF）的增殖反应降低30倍。当细胞在最大刺激浓度的PDGF存在下生长时，cAMP对前体细胞增殖的抑制作用被消除，而cAMP对少突胶质细胞分化的刺激作用依然存在，这表明这两个受cAMP调节的事件是相互独立的。
