McKinnon R D, Smith C, Behar T, Smith T, Dubois-Dalcq M
Laboratory of Viral and Molecular Pathogenesis, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
Glia. 1993 Mar;7(3):245-54. doi: 10.1002/glia.440070308.
We have compared the effects of platelet-derived (PDGF) and basic fibroblast (bFGF) growth factors on the shape, migration, and differentiation of oligodendrocyte progenitor cells, the precursors of myelin-forming cells in the CNS. In the presence of bFGF, oligodendrocyte progenitors purified from rat neonatal brain cultures were stellate, non-motile, and had a morphological complexity of 1.26 +/- 0.03 as measured by fractal dimension (D). These cells expressed transcripts encoding the POU-homeodomain transcription factor Oct-6, but not myelin genes. Upon addition of PDGF, bFGF-treated cells became motile and twofold less complex in shape (D = 1.19 +/- 0.03). These changes occurred within 6 +/- 4 h and were dependent on de novo transcription and translation, but not DNA synthesis. Upon removal of PDGF the cells reverted to their stellate shape (D = 1.26). Removal of both bFGF and PDGF resulted in oligodendrocyte differentiation after 3 days, with a fourfold increase in complexity of shape (D = 1.55 +/- 0.08), loss of Oct-6 transcripts, and gain of myelin transcripts. Thus PDGF is both necessary and sufficient to induce a motile state in progenitor cells growing in the presence of bFGF. Together with our previous data (McKinnon et al.: Neuron 5:603, 1990), our results suggest that bFGF and PDGF may control distinct phases of proliferation and migration of oligodendrocyte progenitor cells in vivo.
我们比较了血小板衍生生长因子（PDGF）和碱性成纤维细胞生长因子（bFGF）对少突胶质前体细胞（中枢神经系统中形成髓磷脂细胞的前体）的形态、迁移和分化的影响。在bFGF存在的情况下，从大鼠新生脑培养物中纯化的少突胶质前体细胞呈星状，无运动能力，通过分形维数（D）测量，其形态复杂性为1.26±0.03。这些细胞表达编码POU同源域转录因子Oct-6 的转录本，但不表达髓磷脂基因。加入PDGF后，经bFGF处理的细胞开始运动，形态复杂性降低两倍（D = 1.19±0.03）。这些变化在6±4小时内发生，并且依赖于从头转录和翻译，但不依赖于DNA合成。去除PDGF后，细胞恢复为星状形态（D = 1.26）。去除bFGF和PDGF两者后，3天后少突胶质细胞发生分化，形态复杂性增加四倍（D = 1.55±0.08），Oct-6转录本丢失，髓磷脂转录本增加。因此，PDGF对于在bFGF存在下生长的前体细胞诱导运动状态既是必要的也是充分的。连同我们之前的数据（麦金农等人：《神经元》5:603，1990），我们的结果表明，bFGF和PDGF可能在体内控制少突胶质前体细胞增殖和迁移的不同阶段。
