Moss C W, Daneshvar M I, Hollis D G
Analytical Chemistry Laboratory, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1993 Mar;31(3):689-91. doi: 10.1128/jcm.31.3.689-691.1993.
Fifteen strains of eugonic, nonoxidative, gram-negative rods isolated primarily from human wounds of the extremities and blood formed a distinct group which was designated Gilardi rod group 1. The phenotypic characteristics of Gilardi rod group 1 were most similar to those of CDC group M-5, with the major difference that nitrite reduction was observed with CDC group M-5. All 15 strains of Gilardi rod group 1 possessed a distinct fatty acid profile which was characterized by large amounts (> 15%) of cis-vaccenic (18:1 omega 7c), palmitic (16:0), myristic (14:0), and lactobacillic (19:0 cyc11,12) acids and moderate amounts (3 to 5%) of lauric (12:0), 3-hydroxylauric (3-OH-12:0), and palmitoleic (16:1 omega 7c) acids. This fatty acid profile is unique compared with the profiles of CDC group M-5 and other bacteria we have tested and is useful for the rapid identification of Gilardi rod group 1 isolates.
主要从人类四肢伤口和血液中分离出的15株生化反应良好、不具氧化能力的革兰氏阴性杆菌形成了一个独特的菌群,被命名为吉拉尔迪杆菌群1。吉拉尔迪杆菌群1的表型特征与疾病控制与预防中心(CDC)的M-5群最为相似,主要区别在于观察到CDC的M-5群能还原亚硝酸盐。吉拉尔迪杆菌群1的所有15个菌株都具有独特的脂肪酸谱,其特征是含有大量(>15%)的顺式vaccenic酸(18:1 ω7c)、棕榈酸(16:0)、肉豆蔻酸(14:0)和乳杆菌酸(19:0 cyc11,12),以及适量(3%至5%)的月桂酸(12:0)、3-羟基月桂酸(3-OH-12:0)和棕榈油酸(16:1 ω7c)。与CDC的M-5群和我们测试过的其他细菌的脂肪酸谱相比,这种脂肪酸谱是独特的,有助于快速鉴定吉拉尔迪杆菌群1的分离株。