Wallace P L, Hollis D G, Weaver R E, Moss C W
Analytical Chemistry Laboratory, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1989 Apr;27(4):735-7. doi: 10.1128/jcm.27.4.735-737.1989.
Fourteen strains of Centers for Disease Control group DF-3 bacteria were examined for cellular fatty acid composition to evaluate their chemical relatedness to known bacterial species and groups. The fatty acids were liberated from whole cells by base hydrolysis, methylated, and analyzed by capillary gas-liquid chromatography. All group DF-3 strains possessed a distinct fatty acid profile which was characterized by large amounts (24%) of 12-methyltetradecanoate (a-C15:0), moderate amounts of saturated iso-branched-chain acids (i-C14:0 and i-C15:0), and small to moderate amounts of both branched- and straight-chain hydroxy acids (3-OH C15:0, i-3-OH C16:0, 3-OH C16:0, and i-3-OH C17:0). This fatty acid profile was unique as compared with the profiles of other bacteria we have previously tested but was most similar to the profiles of Capnocytophaga species.
对14株疾病控制中心DF-3菌群的细菌进行了细胞脂肪酸组成检测,以评估它们与已知细菌种类和菌群的化学相关性。脂肪酸通过碱水解从全细胞中释放出来,进行甲基化,并通过毛细管气液色谱法进行分析。所有DF-3菌群菌株都具有独特的脂肪酸谱,其特征是含有大量(24%)的12-甲基十四烷酸(α-C15:0)、适量的饱和异支链酸(i-C14:0和i-C15:0),以及少量至适量的支链和直链羟基酸(3-OH C15:0、i-3-OH C16:0、3-OH C16:0和i-3-OH C17:0)。与我们之前检测过的其他细菌的脂肪酸谱相比,这种脂肪酸谱是独特的,但与二氧化碳嗜纤维菌属的脂肪酸谱最为相似。