Moss C W, Wallace P L, Hollis D G, Weaver R E
Analytical Chemistry Laboratory, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1988 Mar;26(3):484-92. doi: 10.1128/jcm.26.3.484-492.1988.
We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. All organisms contained ubiquinone with eight isoprene units as the major isoprenolog, but distinct differences were observed in fatty acid composition. Twenty-eight of the original collection of CDC group EO-2 strains were further identified as P. immobilis, EO-2, or EO-3 by distinctive cellular fatty acid profiles, cellular morphology, and pigment production. The cellular fatty acid compositions of M-5 and M-6 were similar but were clearly different from those of other organisms. The genus Acinetobacter was differentiated from other organisms in the study by small amounts of 2-hydroxydodecanoic acid (2-OH-12:0), and P. immobilis was differentiated by small amounts of decanoic acid (10:0) and a branched-chain 17-carbon acid (i-17:0). All Moraxella species were distinguished by small amounts of decanoic acid (10:0) and the absence of i-17:0. M. bovis, M. nonliquefaciens, and some strains of M. lacunata formed a single fatty acid group, while M. osloensis, M. phenylpyruvica, M. atlantae, and other strains of M. lacunata (M. lacunata II) had species-specific fatty acid profiles. O. urethralis differed from Moraxella species by the presence of large amounts (49%) of cis-vaccenic acid (18:1 omega 7c), small amounts (1%) of 3-hydroxyhexadecanoate (3-OH-16:0), and the absence of 10:0 and 3-hydroxydodecanoate (3-OH-12:0). The combined use of chemical data and a small number of conventional tests permitted rapid identification and differentiation of these organisms from each other and from related organisms.
我们测定了疾病控制与预防中心(CDC)EO - 2、M - 5和M - 6组代表性菌株、莫拉克斯氏菌(莫拉克斯氏菌属)、尿道寡源杆菌、不动杆菌属以及嗜冷杆菌的表型特征、细胞脂肪酸组成和类异戊二烯醌含量。所有生物体均含有以八个异戊二烯单元为主的泛醌作为主要类异戊二烯醌,但在脂肪酸组成上观察到明显差异。通过独特的细胞脂肪酸谱、细胞形态和色素产生,CDC EO - 2组原始菌株中的28株被进一步鉴定为嗜冷杆菌、EO - 2或EO - 3。M - 5和M - 6的细胞脂肪酸组成相似,但与其他生物体的明显不同。不动杆菌属在该研究中通过少量的2 - 羟基十二烷酸(2 - OH - 12:0)与其他生物体区分开来,嗜冷杆菌则通过少量的癸酸(10:0)和一种支链十七碳酸(i - 17:0)区分开来。所有莫拉克斯氏菌属物种均通过少量的癸酸(10:0)以及不存在i - 17:0来区分。牛莫拉克斯氏菌、非液化莫拉克斯氏菌和一些腔隙莫拉克斯氏菌菌株形成一个单一的脂肪酸组,而奥斯陆莫拉克斯氏菌、苯丙酮酸莫拉克斯氏菌、大西洋莫拉克斯氏菌和其他腔隙莫拉克斯氏菌菌株(腔隙莫拉克斯氏菌II)具有物种特异性脂肪酸谱。尿道寡源杆菌与莫拉克斯氏菌属物种的不同之处在于存在大量(49%)的顺式- vaccenic酸(18:1 ω7c)、少量(1%)的3 - 羟基十六烷酸酯(3 - OH - 16:0),且不存在10:0和3 - 羟基十二烷酸酯(3 - OH - 12:0)。化学数据与少量常规试验的联合使用使得能够快速鉴定并区分这些生物体彼此之间以及与相关生物体。