Woodall C J, Watt N J, Clements G B
Regional Virus Laboratory, Ruchill Hospital, Glasgow.
J Clin Pathol. 1993 Mar;46(3):276-7. doi: 10.1136/jcp.46.3.276.
The detection of specific RNA species in wax-embedded tissue sections using the polymerase chain reaction (PCR) means that gene expression can be studied and RNA viruses detected in stored histological tissue samples. This technique potentially allows the distribution of gene expression and viral replication to be studied in finely subdivided tissues. A technique is presented that has been used successfully to detect short RNA target sequences (130-420 bases) from proto-oncogene Abelson, human enteroviruses, and the sheep retrovirus Maedi-Visna virus using RNA PCR in single wax sections (20-30 microns). Various tissues were used which had not been deliberately prepared for this purpose. In a simple procedure hot xylene dewaxing is followed by acid phenol extraction of RNA and RNA PCR.
利用聚合酶链反应（PCR）在石蜡包埋组织切片中检测特定RNA种类，意味着可以研究基因表达，并在储存的组织学样本中检测RNA病毒。这项技术有可能用于研究精细细分组织中的基因表达分布和病毒复制情况。本文介绍了一种技术，该技术已成功用于在单张石蜡切片（20 - 30微米）中，通过RNA PCR检测原癌基因阿贝尔森、人肠道病毒以及绵羊逆转录病毒梅迪 - 维斯纳病毒的短RNA靶序列（130 - 420个碱基）。使用了各种并非特意为此准备的组织。在一个简单的操作流程中，先用热二甲苯脱蜡，接着通过酸性酚提取RNA，然后进行RNA PCR。
