酚类化合物的超氧阴离子自由基清除活性。

Superoxide radical scavenging activity of phenolic compounds.

Tsujimoto Y, Hashizume H, Yamazaki M

Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan.

Int J Biochem. 1993 Apr;25(4):491-4. doi: 10.1016/0020-711x(93)90655-x.

Abstract

Superoxide radicals (O2-.) were generated from a hypoxanthin-xanthin oxidase (HPX-XOD) reaction system and detected by the electron spin resonance (ESR) spin-trapping technique. 2. The inhibitory effect on O2-. generation occurred in the order: superoxide dismutase (SOD) > L-ascorbic acid > eugenol > guaiacol > phenol. 3. And the second-order rate constants between O2-. and samples were: SOD 1.7 x 10(9)/M/S, L-ascorbic acid 3.4 x 10(5)/M/S, eugenol 8.3 x 10(3)/M/S, guaiacol 2.5 x 10(3)/M/S and phenol 5.8 x 10(2)/M/S. 4. It was clarified that the phenolic compounds have O2-. scavenging activity.

摘要

超氧阴离子自由基（O2-.）由次黄嘌呤 - 黄嘌呤氧化酶（HPX - XOD）反应系统产生，并通过电子自旋共振（ESR）自旋捕获技术进行检测。2. 对O2-.生成的抑制作用顺序为：超氧化物歧化酶（SOD）> L - 抗坏血酸>丁香酚>愈创木酚>苯酚。3. O2-.与样品之间的二级反应速率常数分别为：SOD 1.7×10(9)/M/S，L - 抗坏血酸3.4×10(5)/M/S，丁香酚8.3×10(3)/M/S，愈创木酚2.5×10(3)/M/S，苯酚5.8×10(2)/M/S。4. 已阐明酚类化合物具有O2-.清除活性。