兔骨骼肌肌钙蛋白-T的一级结构。四个溴化氰片段CB4、CB6和CB7的序列测定。
Primary structure of rabbit skeletal muscle troponin-T. Sequence determination of four cyanogen bromide fragments, CB4, CB6, and CB7.
作者信息
Pearlstone J R, Carpenter M R, Smillie L B
出版信息
J Biol Chem. 1977 Feb 10;252(3):978-82.
PMID:838708
Abstract
The amino acid sequences of four cyanogen bromide (CB) fragments from rabbit skeletal troponin-T have been determined. Fragment CB4 (55 residues) was documented by chymotryptic, thermolytic, tryptic, and 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolenine peptides. A combination of automated sequence analysis, tryptic digestion, and penicillocarboxypeptidase-S treatment was used to assemble fragments CB5 (24 residues), CB7 (8 residues), and the COOH-terminal CB6 (21 residues).
摘要
已测定了来自兔骨骼肌肌钙蛋白-T的四个溴化氰（CB）片段的氨基酸序列。CB4片段（55个残基）通过胰凝乳蛋白酶、嗜热菌蛋白酶、胰蛋白酶和2-（2-硝基苯亚磺酰基）-3-甲基-3'-溴吲哚宁肽进行了记录。采用自动序列分析、胰蛋白酶消化和青霉素羧肽酶-S处理相结合的方法来组装CB5片段（24个残基）、CB7片段（8个残基）以及COOH末端的CB6片段（21个残基）。
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