兔骨骼肌肌钙蛋白-T的一级结构。四个溴化氰片段CB4、CB6和CB7的序列测定。
Primary structure of rabbit skeletal muscle troponin-T. Sequence determination of four cyanogen bromide fragments, CB4, CB6, and CB7.
作者信息
Pearlstone J R, Carpenter M R, Smillie L B
出版信息
J Biol Chem. 1977 Feb 10;252(3):978-82.
PMID:838708
Abstract
The amino acid sequences of four cyanogen bromide (CB) fragments from rabbit skeletal troponin-T have been determined. Fragment CB4 (55 residues) was documented by chymotryptic, thermolytic, tryptic, and 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolenine peptides. A combination of automated sequence analysis, tryptic digestion, and penicillocarboxypeptidase-S treatment was used to assemble fragments CB5 (24 residues), CB7 (8 residues), and the COOH-terminal CB6 (21 residues).
摘要
已测定了来自兔骨骼肌肌钙蛋白-T的四个溴化氰（CB）片段的氨基酸序列。CB4片段（55个残基）通过胰凝乳蛋白酶、嗜热菌蛋白酶、胰蛋白酶和2-（2-硝基苯亚磺酰基）-3-甲基-3'-溴吲哚宁肽进行了记录。采用自动序列分析、胰蛋白酶消化和青霉素羧肽酶-S处理相结合的方法来组装CB5片段（24个残基）、CB7片段（8个残基）以及COOH末端的CB6片段（21个残基）。
相似文献
1
Primary structure of rabbit skeletal muscle troponin-T. Sequence determination of four cyanogen bromide fragments, CB4, CB6, and CB7.
J Biol Chem. 1977 Feb 10;252(3):978-82.
2
Primary structure of rabbit skeletal muscle troponin-T. Purification of cyanogen bromide fragments and the amino acid sequence of fragment CB2.
J Biol Chem. 1977 Feb 10;252(3):971-7.
3
Primary structure of rabbit skeletal muscle troponin-T. Sequence determination of the NH2-terminal fragment CB3 and the complete sequence of troponin-T.
J Biol Chem. 1977 Feb 10;252(3):983-9.
4
5
6
Amino-acid sequence of parvalbumin from rabbit skeletal muscle.
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1309-13. doi: 10.1073/pnas.72.4.1309.
7
The primary structure of actin from rabbit skeletal muscle. Five cyanogen bromide peptides, including the NH2 and COOH termini.
J Biol Chem. 1975 Aug 10;250(15):5897-905.
8
9
Covalent structure of collagen: amino acid sequence of three cyanogen bromide-derived peptides from human alpha 1(V) collagen chain.
Arch Biochem Biophys. 1989 May 15;271(1):120-9. doi: 10.1016/0003-9861(89)90262-2.
10
Primary structure of the polypeptide chain elongation factor Tu from E. coli. I. Amino acid sequence of fragment B.
J Biochem. 1982 Mar;91(3):1047-63. doi: 10.1093/oxfordjournals.jbchem.a133754.
引用本文的文献
1
Structure and function of cardiac troponin C (TNNC1): Implications for heart failure, cardiomyopathies, and troponin modulating drugs.
Gene. 2015 Oct 25;571(2):153-66. doi: 10.1016/j.gene.2015.07.074. Epub 2015 Jul 29.
2
Calmodulin-binding proteins as calpain substrates.
Biochem J. 1989 Sep 15;262(3):693-706. doi: 10.1042/bj2620693.
Zotero 插件