Fujii T, Kawai T, Saito K, Fukushima K, Hasegawa T, Tokunaga M, Yokoyama T
Department of Pathology, Jichi Medical School, Tochigi, Japan.
Acta Pathol Jpn. 1993 Mar;43(3):107-10. doi: 10.1111/j.1440-1827.1993.tb01118.x.
The Epstein-Barr virus-encoded small nuclear RNA, EBER-1, has been shown to be a suitable target for the in situ hybridization detection of EBV in routinely processed tissue specimens. We evaluated the presence of EBV in thymic carcinoma and invasive thymoma using EBER-1 in situ hybridization on formalin-fixed paraffin-embedded tissue sections. EBER-1 expression was demonstrated in a case of lymphoepithelioma-like thymic carcinoma, but was not detectable in other thymic carcinomas including six squamous cell carcinomas, a clear cell carcinoma and seven invasive thymomas. As reported in three previous cases of EBV-associated thymic carcinoma, lymphoepithelioma-like thymic carcinoma was shown to be closely associated with EBV in our series.
爱泼斯坦-巴尔病毒编码的小核RNA（EBER-1）已被证明是在常规处理的组织标本中进行EBV原位杂交检测的合适靶点。我们使用EBER-1原位杂交技术，对福尔马林固定石蜡包埋的组织切片进行检测，以评估胸腺癌和侵袭性胸腺瘤中EBV的存在情况。在一例淋巴上皮瘤样胸腺癌中检测到了EBER-1的表达，但在其他胸腺癌中未检测到，这些胸腺癌包括6例鳞状细胞癌、1例透明细胞癌和7例侵袭性胸腺瘤。正如之前报道的3例EBV相关胸腺癌病例一样，在我们的系列研究中，淋巴上皮瘤样胸腺癌显示与EBV密切相关。
