Gal A A, Unger E R, Koss M N, Yen T S
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia.
Mod Pathol. 1991 Mar;4(2):264-8.
A case of lymphoepithelioma-like carcinoma of the lung is reported in a Chinese patient with serologic evidence of previous Epstein-Barr (EBV) infection. EBV genomes were detected in the tumor using polymerase chain reaction (PCR) and in situ hybridization (ISH) from formalin-fixed, paraffin-embedded tissue blocks. The use of both techniques afforded the exquisite sensitivity of PCR to detect a few copies of EBV DNA with the ability of ISH to precisely localize EBV to the carcinoma, but not to the lymphoid stroma. These findings support a potential oncogenic relationship between EBV and lymphoepithelioma-like carcinoma of the lung.
报告了1例肺淋巴上皮瘤样癌的中国患者，其血清学检查有既往感染EB病毒（EBV）的证据。利用聚合酶链反应（PCR）和原位杂交（ISH）技术，在福尔马林固定、石蜡包埋的组织块中检测到肿瘤组织中的EBV基因组。两种技术的联合使用兼具了PCR检测少量EBV DNA拷贝的高灵敏度以及ISH将EBV精确定位到癌组织而非淋巴间质的能力。这些发现支持了EBV与肺淋巴上皮瘤样癌之间潜在的致癌关系。
