Goodman S B, Rein M S, Hill J A
Fearing Research Laboratory, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
Fertil Steril. 1993 May;59(5):1022-7.
To determine the effects of hysterosalpingography (HSG) contrast media (CM) and chromotubation dye on peritoneal lymphocyte proliferation and macrophage phagocytosis in vitro.
Peritoneal fluid (PF) lymphocytes and macrophages were isolated from 40 subfertile women undergoing diagnostic laparoscopy and 12 fertile women having laparoscopic tubal ligation. Dilutions of renografin, ethiodol, methylene blue, and indigo carmine were added to peritoneal lymphocyte and macrophage cultures. Tissue culture media alone served as control. Lymphocyte proliferation was assessed by hemocytometer counts and 3H-thymidine incorporation. Macrophage function was determined by phagocytosis of fluorescent microspheres.
Peritoneal lymphocyte proliferation and macrophage phagocytosis were significantly inhibited by renografin, ethiodol, methylene blue, and indigo carmine in a dose-dependent manner.
Inhibition of PF immune cell function by HSG CM and chromotubation dye may provide a potential mechanism for fertility enhancement after these diagnostic procedures.
确定子宫输卵管造影术（HSG）造影剂（CM）和输卵管通液染料对体外腹膜淋巴细胞增殖和巨噬细胞吞噬作用的影响。
从40名接受诊断性腹腔镜检查的不育女性和12名接受腹腔镜输卵管结扎术的可育女性中分离出腹膜液（PF）淋巴细胞和巨噬细胞。将泛影葡胺、碘油、亚甲蓝和靛胭脂的稀释液添加到腹膜淋巴细胞和巨噬细胞培养物中。仅用组织培养基作为对照。通过血细胞计数器计数和3H-胸腺嘧啶核苷掺入评估淋巴细胞增殖。通过荧光微球的吞噬作用测定巨噬细胞功能。
泛影葡胺、碘油、亚甲蓝和靛胭脂以剂量依赖方式显著抑制腹膜淋巴细胞增殖和巨噬细胞吞噬作用。
HSG造影剂和输卵管通液染料对PF免疫细胞功能的抑制可能为这些诊断程序后生育力增强提供一种潜在机制。
