鼠伤寒沙门氏菌parE基因的分子特征分析
Molecular characterization of the Salmonella typhimurium parE gene.
作者信息
Springer A L, Schmid M B
机构信息
Department of Molecular Biology, Princeton University, NJ 08544.
出版信息
Nucleic Acids Res. 1993 Apr 25;21(8):1805-9. doi: 10.1093/nar/21.8.1805.
Abstract
The DNA sequence of the wild type S. typhimurium parE gene was determined. The predicted protein has 96.7% amino acid identity with the ParE protein of E.coli, but is 29 amino acids longer, due to an additional basepair in the 3' end of the S. typhimurium gene. Subclones of the S. typhimurium parE gene localized the sites of four heat sensitive mutations within parE. The parE206 and parE374 mutations are identical (Val67-Met) and lie in a highly conserved region corresponding to the ATP binding pocket of GyrB. Two additional heat sensitive mutations were sequenced and predict the following amino acid substitutions: parE377 (Gly399-Ser) and parE493 (Thr583-Pro). All of the heat sensitive mutations lie in regions with strong amino acid homology to GyrB.
摘要
测定了野生型鼠伤寒沙门氏菌parE基因的DNA序列。预测的蛋白质与大肠杆菌的ParE蛋白有96.7%的氨基酸同一性，但由于鼠伤寒沙门氏菌基因3'端有一个额外的碱基对，所以长29个氨基酸。鼠伤寒沙门氏菌parE基因的亚克隆定位了parE内四个热敏感突变的位点。parE206和parE374突变相同（Val67-Met），位于与GyrB的ATP结合口袋相对应的高度保守区域。对另外两个热敏感突变进行了测序，并预测了以下氨基酸取代：parE377（Gly399-Ser）和parE493（Thr583-Pro）。所有热敏感突变都位于与GyrB有强氨基酸同源性的区域。
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