梅毒螺旋体苍白亚种gyrB基因的鉴定与特性分析。

Identification and characterization of the gyrB gene from Treponema pallidum subsp. pallidum.

作者信息

Stamm L V, Greene S R, Barnes N Y

机构信息

Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400, USA.

出版信息

FEMS Microbiol Lett. 1997 Aug 1;153(1):129-34. doi: 10.1111/j.1574-6968.1997.tb10473.x.

DOI:10.1111/j.1574-6968.1997.tb10473.x

PMID:9252582

Abstract

The nucleotide sequence of a DNA gyrase B subunit gene (gyrB) from Treponema pallidum has been determined. Southern blot analysis of T. pallidum chromosomal DNA indicated that this gene is present as a single copy. The organization of genes flanking the gyrB gene is unique in comparison to that of other bacteria. The gyrB gene encodes a 637 amino acid protein whose deduced sequence has a high degree of homology with type-II topoisomerase ATPase subunits (GyrB and ParE). Five type-II topoisomerase motifs, an ATP-binding site (Walker A), and amino acid residues that putatively interact with ATP, are highly conserved in the T. pallidum GyrB protein.

摘要

已确定梅毒螺旋体DNA促旋酶B亚基基因（gyrB）的核苷酸序列。梅毒螺旋体染色体DNA的Southern印迹分析表明，该基因以单拷贝形式存在。与其他细菌相比，gyrB基因侧翼基因的组织方式独特。gyrB基因编码一种637个氨基酸的蛋白质，其推导序列与II型拓扑异构酶ATP酶亚基（GyrB和ParE）具有高度同源性。五个II型拓扑异构酶基序、一个ATP结合位点（沃克A）以及可能与ATP相互作用的氨基酸残基在梅毒螺旋体GyrB蛋白中高度保守。