Otsuji S, Shibata N, Hirota H, Akagami H, Wada A
Department of Cardiology and Pathology, Center for Adult Diseases, Osaka, Japan.
Jpn Circ J. 1993 Apr;57(4):335-43. doi: 10.1253/jcj.57.335.
We examined the effects of dietary supplementation with eicosapentaenoic acid (EPA) on experimental myocardial infarction in dogs. Twenty-five dogs were fed standard diets, 10 of which were supplemented with EPA-ester (100 mg/kg body weight/day) for 8 weeks, while 15 served as controls. After ingesting EPA for 8 weeks, the ratio of EPA to arachidonic acid (AA) in platelet cell membranes significantly increased (from 0.033 to 0.105; p < 0.01). The chemotactic response of neutrophils to leukotriene B4 (LTB4) was reduced in the EPA group (34% reduction at 10(-6) M LTB4, p < 0.01). Also in the EPA group, the amount of 12-hydroxyeicosatetraenoic acid, one of the chemotactic products of AA in infarcted myocardium, was reduced to 40% (p < 0.05). EPA treatment resulted in significant reduction in the ultimate size of the infarcted area. Contractile function of infarcted myocardium was well-preserved in the EPA group. Myeloperoxidase activity, an indication of the infiltration of neutrophils into the infarcted myocardium, was less in the EPA group than in the controls (0.68 +/- 0.25 U/0.1 gr. vs 1.22 +/- 0.55 U/0.1 gr., p < 0.05). Therefore, we conclude that dietary supplementation with EPA attenuates ischemic myocardial damage through inhibition of neutrophilic infiltration into the infarcted myocardium.
我们研究了膳食补充二十碳五烯酸（EPA）对犬实验性心肌梗死的影响。25只犬喂食标准饮食，其中10只补充EPA酯（100毫克/千克体重/天），持续8周，15只作为对照。摄入EPA 8周后，血小板细胞膜中EPA与花生四烯酸（AA）的比例显著增加（从0.033增至0.105；p<0.01）。EPA组中性粒细胞对白三烯B4（LTB4）的趋化反应降低（在10^(-6)M LTB4时降低34%，p<0.01）。同样在EPA组，梗死心肌中AA的趋化产物之一12-羟基二十碳四烯酸的量减少至40%（p<0.05）。EPA治疗导致梗死面积最终大小显著减小。EPA组梗死心肌的收缩功能得到良好保留。EPA组中髓过氧化物酶活性（中性粒细胞浸润梗死心肌的一个指标）低于对照组（0.68±0.25 U/0.1克对1.22±0.55 U/0.1克，p<0.05）。因此，我们得出结论，膳食补充EPA通过抑制中性粒细胞浸润梗死心肌来减轻缺血性心肌损伤。
