Russo F D, Slauch J M, Silhavy T J
Department of Genetics, University of Washington, Seattle 98195.
J Mol Biol. 1993 May 20;231(2):261-73. doi: 10.1006/jmbi.1993.1281.
OmpR is a member of a family of bacterial transcriptional regulators whose activity is controlled by phosphorylation. It regulates the transcription of two genes, serving as an activator of ompC, and as both an activator and a repressor of ompF. A previously isolated collection of ompR mutations was analyzed for the effect of each on the expression of both genes simultaneously. The results of this analysis indicate that the activation, repression, and DNA binding functions of OmpR can be disrupted independently, and that mutations interfering with each of these functions cluster within the sequence of the OmpR protein. The nature of these mutations is discussed in terms of the mechanisms by which OmpR regulates transcription, and potentially similar mechanisms operating within closely related response regulators.
OmpR是细菌转录调节因子家族的成员，其活性受磷酸化作用控制。它调节两个基因的转录，作为ompC的激活剂，以及ompF的激活剂和抑制剂。对先前分离得到的一组ompR突变体进行分析，以研究每个突变对这两个基因表达的同时影响。分析结果表明，OmpR的激活、抑制和DNA结合功能可以独立被破坏，并且干扰这些功能的突变聚集在OmpR蛋白序列内。根据OmpR调节转录的机制以及在密切相关的应答调节因子中可能起作用的类似机制，对这些突变的性质进行了讨论。
