Santiago L A, Osato J A, Liu J, Mori A
Department of Neuroscience, Okayama University Medical School, Japan.
Neurochem Res. 1993 Jun;18(6):711-7. doi: 10.1007/BF00966786.
This study describes, using electron spin resonance spectrometry/spin trapping technique, the increase superoxide dismutase (SOD) activity in the mitochondrial and cytosolic fraction of the cortex, midbrain, pons-medulla oblongata and cerebellum, and in thiobarbituric acid-reactive substances (TBARS) in the cortex, cerebellum and hippocampus of the aged rats. The results show that corresponding to the increased life span and improved physical conditions observed after peroral long-term treatment with Bio-catalyzer, a commercial natural fermented health food supplement marketed in Japan and in the Philippines and earlier reported to be a hydroxyl radical scavenger with weaker scavenging activity on superoxide radical (O-2), SOD which is involved in the metabolic degradation of O-2 was further increased, whereas TBARS decreased. These findings suggest that the increased SOD activity in the brain as a defense mechanism against age-related accumulation of reactive oxygen species, in particular superoxide radicals, was enhanced with Bio-catalyzer treatment while age-related peroxidation of neuronal membrane, as measured by TBARS, was decreased.
本研究运用电子自旋共振光谱法/自旋捕获技术，描述了老年大鼠大脑皮层、中脑、脑桥-延髓和小脑线粒体及胞质部分中超氧化物歧化酶（SOD）活性的增加，以及大脑皮层、小脑和海马中硫代巴比妥酸反应性物质（TBARS）的变化。结果表明，与长期口服生物催化剂（一种在日本和菲律宾销售的商业天然发酵健康食品补充剂，此前报道其为羟自由基清除剂，对超氧阴离子自由基（O₂）的清除活性较弱）后观察到的寿命延长和身体状况改善相对应，参与O₂代谢降解的SOD进一步增加，而TBARS减少。这些发现表明，生物催化剂处理增强了大脑中SOD活性的增加，这是一种针对与年龄相关的活性氧特别是超氧阴离子自由基积累的防御机制，同时通过TBARS测量的与年龄相关的神经元膜过氧化作用降低。
