Ueda Y, Komatsu M, Hiramatsu M
Division of Medical Science, Yamagata Technopolis Foundation, Japan.
Neurochem Res. 1996 Aug;21(8):909-14. doi: 10.1007/BF02532340.
The free radical scavenging activity of the Japanese herbal medicine, Toki-Shakuyaku-San (TJ-23; TSUMURA & Co., Tokyo, Japan), was examined using electron spin resonance (ESR) spectrometry. TJ-23 scavenged 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), superoxide (O2-), and hydroxyl radicals (.OH) dose-dependently. It also diminished carbon centered radicals (.C) generated by oxidative stress and inhibited thiobarbituric acid-reactive substances (TBARS) formation in mouse cortex homogenate. In addition, the effect of TJ-23 on the concentration of neurotransmitters and TBARS formation, and superoxide dismutase (SOD) activity in the cortex, hippocampus and striatum of the aged rat brain was studied. The concentrations of the metabolites of monoamines, glutamate and glutamine were decreased by 4 weeks of oral administration of TJ-23. The SOD activity of mitochondrial fraction was increased and TBARS formation was significantly suppressed. These results suggest that TJ-23 has an antioxidant action and would have a prophylactic effect against free radical-mediated neurological diseases associated with aging.
采用电子自旋共振（ESR）光谱法检测了日本草药柴胡桂枝汤（TJ - 23；津村株式会社，东京，日本）的自由基清除活性。TJ - 23对1,1 - 二苯基 - 2 - 苦基肼自由基（DPPH）、超氧阴离子（O₂⁻）和羟基自由基（·OH）的清除呈剂量依赖性。它还能减少氧化应激产生的碳中心自由基（·C），并抑制小鼠皮质匀浆中硫代巴比妥酸反应性物质（TBARS）的形成。此外，研究了TJ - 23对老年大鼠大脑皮质、海马和纹状体中神经递质浓度、TBARS形成以及超氧化物歧化酶（SOD）活性的影响。口服TJ - 23 4周后，单胺、谷氨酸和谷氨酰胺的代谢物浓度降低。线粒体部分的SOD活性增加，TBARS形成受到显著抑制。这些结果表明，TJ - 23具有抗氧化作用，可能对与衰老相关的自由基介导的神经疾病具有预防作用。
