Onda T, Kanda T, Zanma S, Yasugi T, Watanabe S, Kawana T, Ueda K, Yoshikawa H, Taketani Y, Yoshiike K
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
Int J Cancer. 1993 Jun 19;54(4):624-8. doi: 10.1002/ijc.2910540417.
Occurrence of the antibodies against human papillomavirus (HPV) 16 proteins E4 and E7 is specifically but independently associated with cervical cancer. To correlate HPV DNA and antibody data, we examined the biopsy specimens and sera, by polymerase chain reaction (PCR) and by ELISA, respectively, from 51 patients with cervical cancer (including 3 recurrent cases) and 22 with cervical intra-epithelial neoplasia. Consensus primers for the L1 region were used for PCR and bacterially expressed, purified fusion protein HPV-16 E4 and non-fusion protein HPV-16 E7 were used for ELISA. HPV-16 DNA and other HPV types were detected in 17 and 25, respectively, out of 51 cases of cervical cancer. Ten out of the 17 HPV-16-DNA-positives were positive either for anti-E4 or for anti-E7: positivities for anti-E4, for anti-E7, and for both were 6/17, 5/17 and 1/17 respectively. Three anti-E7-positives consisted of those for HPV-33, -52 and -58 DNA, suggesting that limited cross-reaction occurred between the HPV types. Among the HPV-16-DNA-positive cases of cancer, lymph-node or distant metastasis was recorded more frequently in the seropositives than in the seronegatives. Our results show that the HPV-16 anti-E4 or anti-E7 occurs in some, but not in all, of the HPV-16-DNA-positive cases, and support the hypothesis that the presence of the HPV-16 antibodies can be used as a marker for possible metastasis.
抗人乳头瘤病毒（HPV）16型蛋白E4和E7抗体的出现与宫颈癌存在特异性但独立的关联。为了关联HPV DNA和抗体数据，我们分别通过聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）检测了51例宫颈癌患者（包括3例复发病例）和22例宫颈上皮内瘤变患者的活检标本和血清。用于PCR的L1区域的共有引物以及用于ELISA的细菌表达、纯化的融合蛋白HPV-16 E4和非融合蛋白HPV-16 E7。51例宫颈癌病例中分别检测到17例HPV-16 DNA和25例其他HPV类型。17例HPV-16 DNA阳性患者中有10例抗E4或抗E7呈阳性：抗E4阳性、抗E7阳性以及两者均阳性的比例分别为6/17、5/17和1/17。3例抗E7阳性患者的HPV DNA类型为HPV-33、-52和-58，提示HPV各型之间存在有限的交叉反应。在HPV-16 DNA阳性的癌症病例中，血清阳性患者发生淋巴结或远处转移的频率高于血清阴性患者。我们的结果表明，HPV-16抗E4或抗E7在部分而非全部HPV-16 DNA阳性病例中出现，并支持HPV-16抗体的存在可作为可能发生转移的标志物这一假说。
