Nakagawa Mayumi, Viscidi Raphael, Deshmukh Ian, Costa Maria Da, Palefsky Joel M, Farhat Sepideh, Moscicki Anna-Barbara
Departments of Laboratory Medicine, Schools of Medicine and Dentistry, University of California at San Francisco, San Francisco, California 94143-0134, USA.
Clin Diagn Lab Immunol. 2002 Jul;9(4):877-82. doi: 10.1128/cdli.9.4.877-882.2002.
The time course of cell-mediated and humoral immune responses was elucidated in eight women with human papillomavirus type 16 (HPV-16) infection by performing serial HPV-16 E6 and E7 cytotoxic T-lymphocyte (CTL) assays and HPV-16 virus-like particle (VLP) antibody analyses. Four subjects had a single incident of HPV-16 DNA detection, and four subjects had two periods of HPV-16 DNA detection. In two of the women in the latter group, the second episode of HPV-16 detection occurred in the presence of high titers of HPV-16 VLP antibody, bringing into question the protective role of humoral immunity in preventing repeated infection. However, all four subjects rapidly became HPV-16 DNA negative following the second detection of HPV-16 DNA, suggesting the presence of immunological memory. In addition, one subject rapidly became negative for HPV-16 DNA despite having no evidence of CTL or VLP antibody response prior to the second HPV-16 DNA detection, suggesting the presence of immunological responses at an undetectable level. Overall, seven of eight subjects (88%) had detectable HPV-16 E6 and/or E7 CTL responses and seven of eight women (88%) had detectable HPV-16 VLP antibody responses.
通过进行系列人乳头瘤病毒16型（HPV - 16）E6和E7细胞毒性T淋巴细胞（CTL）检测以及HPV - 16病毒样颗粒（VLP）抗体分析，阐明了8名感染HPV - 16的女性细胞介导免疫反应和体液免疫反应的时间进程。4名受试者有单次HPV - 16 DNA检测事件，4名受试者有两个阶段的HPV - 16 DNA检测。在后一组中的2名女性中，第二次HPV - 16检测事件发生在高滴度HPV - 16 VLP抗体存在的情况下，这使人质疑体液免疫在预防重复感染中的保护作用。然而，所有4名受试者在第二次检测到HPV - 16 DNA后迅速变为HPV - 16 DNA阴性，提示存在免疫记忆。此外，1名受试者在第二次HPV - 16 DNA检测前虽无CTL或VLP抗体反应的证据，但HPV - 16 DNA迅速变为阴性，提示存在无法检测到水平的免疫反应。总体而言，8名受试者中有7名（88%）有可检测到的HPV - 16 E6和/或E7 CTL反应，8名女性中有7名（88%）有可检测到的HPV - 16 VLP抗体反应。
