Nakagawa M, Stites D P, Farhat S, Judd A, Moscicki A B, Canchola A J, Hilton J F, Palefsky J M
Department of Laboratory Medicine, School of Medicine, University of California at San Francisco 94143-0134, USA.
Clin Diagn Lab Immunol. 1996 Mar;3(2):205-10. doi: 10.1128/cdli.3.2.205-210.1996.
The incidence of human papillomavirus (HPV)-related cervical intraepithelial neoplasia (CIN) and cervical cancer is increased with immunodeficiency, but the role of immune response, including cell-mediated immunity, in disease prevention is not well understood. In this study, T-cell proliferative responses to six synthetic peptides with predicted immunogenic determinants from the HPV-16 E4, E6, E7, and L1 open reading frames were analyzed in 22 sexually active women with new-onset CIN and 65 sexually active women without cervical disease, characterized by cytology, colposcopy, and HPV testing. T-cell proliferative responses were demonstrated to all six HPV-16 peptides. Although not statistically significant, rates of reactivity to E6 (24-45) were higher among sexually active women without disease (26%) than among women with current CIN (7%), as was the overall number of peptides stimulating a response. Women with CIN may not respond to selected HPV antigens as well as women without disease do.
人乳头瘤病毒（HPV）相关的宫颈上皮内瘤变（CIN）和宫颈癌的发病率会因免疫缺陷而升高，但包括细胞介导免疫在内的免疫反应在疾病预防中的作用尚未完全明确。在本研究中，对22例新发CIN的性活跃女性和65例无宫颈疾病的性活跃女性（通过细胞学、阴道镜检查和HPV检测进行特征分析），分析了其对来自HPV-16 E4、E6、E7和L1开放阅读框的六种具有预测免疫原性决定簇的合成肽的T细胞增殖反应。结果显示，所有六种HPV-16肽均能引发T细胞增殖反应。虽然无统计学意义，但无疾病的性活跃女性对E6（24 - 45）的反应率（26%）高于现患CIN的女性（7%），刺激反应的肽的总数亦是如此。患有CIN的女性对特定HPV抗原的反应可能不如无疾病的女性。
