Vandenbark A A, Burger D R, Dreyer D L, Daves G D, Vetto R M
J Immunol. 1977 Feb;118(2):636-41.
The biologically active fraction of human transfer factor (Sephadex G-25, fraction III) has been separated by exclusion chromatography into two component fractions (IIIa and IIIb). Fraction IIIa transferred donor reactivities to PPD or KLH into 9 of 9 recipients. Fraction IIIb did not transfer donor reactivities (0 of 8 transfers) but did produce both antigen-independent and antigen-dependent dermal reactions in recipients. Fractionation by electrofocusing and high pressure reverse phase chromatography revealed that IIIa has a major UV254 absorbing component and that fraction IIIb has at least three major and three minor UV absorbing components. The major component of IIIa was isolated by preparative electrofocusing, had an isoelectric point of 1.6, and transferred KLH reactivity to four of five recipients.
人转移因子的生物活性部分（葡聚糖凝胶G - 25，组分III）通过排阻色谱法分离为两个组分部分（IIIa和IIIb）。组分IIIa将供体对PPD或KLH的反应性转移给9名受者中的9名。组分IIIb未转移供体反应性（8次转移中有0次），但确实在受者中产生了抗原非依赖性和抗原依赖性皮肤反应。通过等电聚焦和高压反相色谱法分级分离显示，IIIa有一个主要的吸收UV254的成分，而组分IIIb至少有三个主要和三个次要的吸收UV的成分。IIIa的主要成分通过制备性等电聚焦分离得到，其等电点为1.6，并将KLH反应性转移给五名受者中的四名。
